Association Between Mitochondrial DNA Content and Risk of Coronary Heart Disease

Association Between Mitochondrial DNA Copy Number in Peripheral Blood Cells and Risk of Developing Coronary Heart Disease

Status

Completed

Phases

Unknown

Study type

Observational

Source

ClinicalTrials.gov

Registry ID

NCT02500823

Enrollment

400

Registered

2015-07-17

Start date

2015-04-30

Completion date

2015-11-30

Last updated

2016-05-17

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Coronary Heart Disease

Keywords

Mitochondrial DNA copy number, Peripheral blood cells

Brief summary

Compelling epidemiological evidence indicates that alterations of mitochondrial DNA, including mutations and abnormal content of mitochondrial DNA (mtDNA), are associated with the initiation and development of cardiovascular disease. This study was undertaken to investigate whether mtDNA copy number in peripheral blood cells could be used as a risk predictor for coronary heart disease.

Interventions

None listed

Study design

Observational model

CASE_CONTROL

Time perspective

RETROSPECTIVE

Eligibility

Sex/Gender

ALL

Age

18 Years to 85 Years

Healthy volunteers

Yes

Inclusion criteria

1. History of documented myocardial infarction; 2. Prior coronary revascularization intervention (coronary artery bypass graft surgery or percutaneous coronary intervention); 3. The presence of ≥ 50% stenosis in one or more coronary arteries identified during cardiac catheterization;

Exclusion criteria

1. History of malignancy or end-stage renal disease; 2. Blood transfusion within one month or prior bone marrow transplantation 3. Patients who reluctant to sign informed consent

Design outcomes

Primary

Measure	Time frame	Description
Relative copy number of mitochondrial DNA	From date of percutaneous coronary intervention until the date of discharging from hospital, assessed up to 5 days	The ratio of mitochondrial DNA copy number to hemoglobin copy number was calculated for each sample from standard curves. After that, the ratio for each sample was normalized to a calibrator DNA in order to standardize between different runs, and then defined as the measurement of relative mtDNA copy number.Relative copy number of Mitochondrial DNA were measured in CHD patients in comparison to control group.

Countries

China

Outcome results