A Comparison Between Conventional Surgery and Piezosurgery

A Histo-immunological Comparative Study of Bone Cutting by Conventional Surgery and Piezosurgery on the Secretion of Heat Shock Protein 70 (HSP70) and on the Bone Cells

Status

Completed

Phases

Study type

Interventional

Source

ClinicalTrials.gov

Registry ID

NCT02495207

Enrollment

Registered

2015-07-13

Start date

2014-06-30

Completion date

2017-04-14

Last updated

2017-07-27

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Third Molars Extraction

Brief summary

Evaluation of the effect of conventional and piezo-surgery on the secretion of Heat Shock Protein (HSP70) and on the vial bone cells at the peripheral bone cut sides after the removal of impacted mandibular third molars on both sides of the jaw.

Detailed description

The study is about measuring the amount of HSP70 using Elisa kit for detecting HSP70 and mason trichrome for histological findings in which the study samples are patients who have bilateral impacted mandibular third molar to be extracted with bone removal. First we collect bone sample for mason trichrome with either method then we collect the second samples after removing most of the bones cover the impacted third molar for checking HSP70 levels. Then the bone samples are treated using a special protocol to eliminate the protein from bone samples for the Elisa analysis. The other bone samples are going to be studied under the microscope.

Interventions

DEVICEPiezosurgery

This intervention is dependent on use of piezosurgery to conduct the required bone cuts to remove third molars.

Study design

Allocation

RANDOMIZED

Intervention model

PARALLEL

Primary purpose

TREATMENT

Masking

NONE

Eligibility

Sex/Gender

ALL

Age

16 Years to 35 Years

Healthy volunteers

Inclusion criteria

* bilateral identical impacted third molar that need at least 3 mm of bone removal

Exclusion criteria

* Pregnancy * Smokers * Infectious diseases * Infections around third molars (i.e. pericoronitis). * Alcoholic patients

Design outcomes

Primary

Measure	Time frame	Description
Levels of Heat Shock Protein 70	Five minutes before third molar removal with the last removed layer of bone	This will be measured using ELISA methodology

Secondary

Measure	Time frame	Description
Percentage of viable bone cells	This variable will be measured from the specimens taken within the first 15 minutes of bone cutting to remove third molars	Percentage of viable bone cells will be calculated based on the counts of viable bone cells observed under the microscope.

Countries

Syria

Outcome results

None listed

Source: ClinicalTrials.gov · Data processed: Feb 4, 2026