Healthy
Conditions
Keywords
PYY, Degradation, kinetics, men
Brief summary
Peptide YY (PYY) is a gastrointestinal hormone released from the enteroendocrine cell upon food intake. The N-terminal truncated form, PYY3-36, exerts anorexic effects. In this study we want to investigate the kinetics of PYY1-36 and PYY3-36 and to examine whether a C-terminally degraded metabolite, PYY3-34, is formed after infusion of PYY.
Interventions
BIOLOGICALPYY1-36 infusion
Infusion of 1.6 pmol/kg/min PYY1-36 for 2 hrs.
BIOLOGICALPYY1-36 + sitagliptin
Infusion of 1.6 pmol/kg/min PYY1-36 for 2 hrs. Two doses of 100 mg sitagliptin are administered 10 and 1 hour before start of infusion, respectively.
BIOLOGICALPYY3-36 infusion
0.8 pmol/kg/min infusion of PYY3-36.
BIOLOGICALplacebo
Infusion of saline for 2 hrs.
Sponsors
University of Copenhagen
Hvidovre University Hospital
Study design
Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
BASIC_SCIENCE
Masking
SINGLE (Subject)
Eligibility
Sex/Gender
MALE
Age
18 Years to 50 Years
Healthy volunteers
Yes
Inclusion criteria
* Healthy * Normal BMI (18.5-25kg/m2)
Exclusion criteria
* Diabetes Mellitus * Gastric bypass * Non-caucasian * Height changes \>3 kg within last two months * Hemoglobin \< 7.6 mmol/l * Chronic disease * Smoking * Regularly use of medicine * Drug abuse
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| PYY3-34 concentration | 0-240 min |
| Half-lives of PYY1-36 and PYY3-36 | 0-240 min |
Other
| Measure | Time frame | Description |
|---|---|---|
| Appetite and nausea scores | 0-240 min | Between infusion differences in VAS scores |
| Blood pressure | 0-240 min | Between infusion differences in blood pressure |
| Blood glucose | 0-240 min | Between infusion differences in blood glucose |
| Heart rate | 0-240 min | Between infusion differences in heart rate |
Countries
Denmark
Outcome results
None listed