Healthy
Conditions
Keywords
Spices, Functional food, Cardiometabolic risk, Metabolic syndrome, Inflammation, Peripheral blood mononuclear cell, Acute
Brief summary
This study aims to investigate the effect of spices on gene expression of pathways associated with glucose homeostasis, oxidative stress and inflammation, in the postprandial phase in healthy human subjects.
Interventions
OTHERFat challenge breakfast
In this intervention, subjects are asked to consume 200 ml control drinks (non-energy flavored water). Following 10 min. beverage consumption, subjects will be given standardized high fat breakfast corresponding to % Energy of Carbohydrate:Fat:Prot / 20:60:20
OTHERFat challenge breakfast+spices
In this intervention, subjects are asked to consume 200 ml spices drinks (non-energy flavored water + spices extract). Following 10 min. beverage consumption, subjects will be given standardized high fat breakfast corresponding to % Energy of Carbohydrate:Fat:Prot / 20:60:20
OTHERCarbohydrate challenge breakfast
In this intervention, subjects are asked to consume 200 ml control drinks (non-energy flavored water). Following 10 min. beverage consumption, subjects will be given standardized high carbohydrate breakfast corresponding to % Energy of Carbohydrate:Fat:Prot / 60:20:20
OTHERCarbohydrate challenge breakfast+spices
In this intervention, subjects are asked to consume 200 ml spices drinks (non-energy flavored water + spices extract). Following 10 min. beverage consumption, subjects will be given standardized high carbohydrate breakfast corresponding to % Energy of Carbohydrate:Fat:Prot / 60:20:20
Sponsors
Food for Health Science Centre, Lund University
Lund University
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
SINGLE (Subject)
Eligibility
Sex/Gender
ALL
Age
18 Years to 40 Years
Healthy volunteers
Yes
Inclusion criteria
* Healthy males and females * BMI 20-28 kg/m2 * Must be able to accept spices * Agreed and signed informed consent
Exclusion criteria
* Below 18 years old * Uncomfortable speaking English and/or difficulties in understanding spoken English * Smoking or using snuss * Vegetarian or vegan * Stressed by venous blood sampling or previous experience of being difficult to be cannulated * Receiving any drug treatment that may influence the study outcomes * Pregnancy or breastfeeding
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Change from Baseline (0 hr) in untargeted PBMC gene expression profile at 2 hr and 4 hr | Prior to the initial intervention at 0 hr and after intervention at 2 hr and 4 hr. | The venous blood samples will be taken for isolation of peripheral blood mononuclear cells. Extracted total RNA will then be used for running RNA-seq/whole-transcriptome shotgun sequencing (Illumina, San Diego, CA, USA) |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Subjective appetite ratings using standard subjective 100 mm VAS | Prior to the initial intervention at 0 min and after intervention at 15, 30, 45, 60, 90, 120, 180 and 240 min. | Appetite will be assessed using standard subjective 100 mm VAS (visual analogue scale) at time intervals throughout each visit |
| Metabolic biomarkers | Prior to the initial intervention at 0 min and after intervention at 15, 30, 45, 60, 90, 120, 180 and 240 min. | The capillary blood samples will be taken for blood glucose analysis. The venous blood samples will be taken for further assessment of insulin, triacylglycerol, NEFA and inflammatory biomarkers (i.e. IL-6, IL-8, CRP) |
| Metabolite profile | Prior to the initial intervention at 0 min and after intervention at 15, 30, 45, 60, 90, 120, 180 and 240 min. | Metabolite profile will be analyzed using Liquid Chromatography-Mass Spectrometry based methods |
Countries
Sweden
Outcome results
None listed