Healthy, Increased Metabolic Requirement
Conditions
Brief summary
Protein requirements in active individuals have been suggested to be greater than the current recommended dietary allowance (RDA). Nutritional requirements for dietary amino acids in adults have traditionally been determined utilizing the nitrogen balance technique, which is prone to underestimating protein requirements. As a result, there is a need to re-evaluate recommendations in order to characterize how dietary amino acid needs may be modulated by physical activity. Recent studies using the minimally invasive indicator amino acid oxidation (IAAO) technique have suggested that protein requirements in young men are at least 50% higher than the RDA based on nitrogen balance data. The purpose of this study is to measure the protein requirement in active, young adult males using the IAAO technique in the presence of a variable intensity exercise stimulus.
Interventions
DIETARY_SUPPLEMENTAmino Acid Intake
Amino acid intakes will be varied at 0.2-2.6 g/kg/day
Sponsors
Ajinomoto Co., Inc.
University of Toronto
Study design
Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
MALE
Age
18 Years to 35 Years
Healthy volunteers
Yes
Inclusion criteria
* Healthy, active, young adult males who regularly participate in moderate-vigorous activity 5x per week, for a minimum of 1 hour * A minimum score of 10 on the Beep Test (a measure of aerobic fitness)
Exclusion criteria
* Inability to meet health and physical activity guidelines according to the physical activity readiness questionnaire (PAR-Q+) * Inability to adhere to any of the protocol guidelines (i.e. alcohol, caffeine consumption) * Regular tobacco use * Illicit drug use
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| 13CO2 (carbon dioxide) excretion (μmol/kg/h) | 4 hours / study day |
Secondary
| Measure | Time frame |
|---|---|
| 13C-Phenylalanine Oxidation (μmol/kg/h) | 4 hours / study day |
Outcome results
None listed