Healthy, Increased Metabolic Requirement
Conditions
Brief summary
Protein requirements in individuals who participate in endurance-based exercise training have been suggested to be greater than the current recommended dietary allowance (RDA). Nutritional requirements for dietary amino acids in adults have traditionally been determined utilizing the nitrogen balance technique, which is prone to underestimating protein requirements. As a result, there is a need to re-evaluate recommendations in order to characterize how dietary amino acid needs may be modulated by physical activity. Recent studies using the minimally invasive indicator amino acid oxidation (IAAO) technique have suggested that protein requirements in young men are at least 50% higher than RDA based on nitrogen balance data. Therefore, the purpose of this study is to measure the protein requirement in endurance trained adults using the IAAO technique.
Interventions
amino acids intakes will be varied at 0.2-2.8 g/kg/day
Sponsors
Study design
Eligibility
Inclusion criteria
* Healthy, male, endurance-trained participants who regularly more than 40 km/week * Ability to perform the exercise stimulus (20 km run) on metabolic trial.
Exclusion criteria
* Inability to meet health and physical activity guidelines according to the physical activity readiness questionnaire (PAR-Q+) * Inability to adhere to any of the protocol guidelines (i.e. alcohol, caffeine consumption) * Regular tobacco use * Illicit drug use
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| 13CO2 (carbon dioxide) excretion (μmol/kg/h) | 4 hours / study day |
Secondary
| Measure | Time frame |
|---|---|
| [13C]phenylalanine oxidation (μmol/kg/h) | 4 hours/study day |