Celiac Disease
Conditions
Keywords
gluten, antigen-specific T cells, transcriptome
Brief summary
Up till 30 participants with celiac disease on a glutenfree diet are asked to consume gluten containing cookies or bread for 3 days. Questionnaires and sampling of blood is done before, during and after.
Detailed description
Before and 6 days after start of gluten challenge, blood samples will be collected and analysed by using HLA-tetramers to identify and purify CD4+ gluten-specific T cells by flow cytometry. T cells will undergo transcriptome analysis to get an insight in signalling of antigen-specific cells. Other cell populations like CD8+ T cells and γδ T cells will also be analyzed by flow cytometry.
Interventions
DIETARY_SUPPLEMENTGluten challenge
Challenge done in the form of a gluten containing cookie once daily, or up to four slices of regular bread daily.
Sponsors
Stiftelsen Helse og Rehabilitering
Helse Sor-Ost
Oslo University Hospital
Study design
Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
18 Years to 80 Years
Healthy volunteers
No
Inclusion criteria
* Celiac disease confirmed by biopsy * Age 18 - 80 years * Gluten free diet last 6 months * Subject has received information and signed the informed consent form
Exclusion criteria
* Pregnant or breast feeding * Probability of participant becoming pregnant (f.ex. by not using adequate sexual prevention by woman in fertile age) * Drugs influencing immune system used last 3 months * Current infectious disease of moderate or high severity * Other chronic active intestinal disease * Serious reaction on small amounts of gluten ingested
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Transcriptome of gluten-specific T cells | Day 6 | Transcriptional differences of glutenspecific vs. unspecific T cells |
Countries
Norway
Outcome results
None listed