Preliminary Study to the Manufacturing of a Non-stick Membrane for Surgery Using Patient's Own Cells (MESO-PATCH)

Non-stick Patch of Mesothelial Cells in a Hydrogel Matrix : Exploratory Analysis Assessing the Morphotype and Mesothelial Phenotype After Culture From 2 Cells Sources of Human Origin.

Status

Terminated

Phases

Unknown

Study type

Interventional

Source

ClinicalTrials.gov

Registry ID

NCT02439047

Acronym

MESO-PATCH

Enrollment

Registered

2015-05-08

Start date

2015-06-01

Completion date

2016-11-01

Last updated

2026-03-16

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Cancer

Keywords

reconstructive surgery, abdominal surgery

Brief summary

The objective of this study, which will be realised in vitro on human samples, is to test 2 cell sources isolated from peritoneum for adult mesothelial cells (peritoneal samples) and from adipose tissue for the adipose stem cells (adipose tissue samples). The latter will be differentiated towards the mesothelial phenotype and morphotype. The 2 routes will be compared and the most satisfactory one will be chosen to make a non-stick patch.

Detailed description

* Patient information and collection of a signed informed consent * Clinical data collection: surgery date, age, weight, height, medical and surgical history * Prospective collection of peritoneal and adipose biopsies of patients during their surgery at the Oscar Lambret Center (Dr L. BRESSON) * Transportation of the samples to the 1008 INSERM Unit. * Isolation of cells from the samples : mesothelial cells from the peritoneum and adipose stem cells from adipose tissue * Cell culture and expansion of mesothelial cells; and cell culture and differentiation of adipose stem cells * Identification by the morphotype and phenotype of mesothelial cells * Comparison of the results according to the cell source, peritoneum or adipocyte, to the purity of cells and to the efficacy of the expansion

Interventions

PROCEDUREBiological samples collection

-Peritoneal samples : will be collected during abdominal surgery A biopsy from 1 to 2 cm2 will be performed in healthy peritoneum \- Adipose tissue samples : will be collected during surgery for breast reconstruction

Study design

Allocation

Intervention model

SINGLE_GROUP

Primary purpose

BASIC_SCIENCE

Masking

NONE

Eligibility

Sex/Gender

ALL

Age

18 Years to No maximum

Healthy volunteers

Inclusion criteria

* Aged more than 18 years old, * Planned surgery For peritoneal samples : abdominal surgery (laparotomy or coelioscopy) For adipose tissue samples : breast reconstructive surgery (lipomodelage) * Informed and signed consent

Exclusion criteria

* Pregnant or breastfeeding woman * Patient under guardianship or tutorship * For peritoneal samples : previous abdomino-pelvic radiotherapy or abdominal surgery ; peritoneal carcinosis

Design outcomes

Primary

Measure	Time frame	Description
Morphotype/Phenotype expression	1 year	Mesothelial morphotype and phenotype expression of the 2 types of cells after culture

Countries

France

Contacts

PRINCIPAL_INVESTIGATORLucie BRESSON, MD

Centre Oscar Lambret

STUDY_DIRECTORFeng Chai, MD

INSERM U1008

Outcome results

None listed

Source: ClinicalTrials.gov · Data processed: Mar 17, 2026