In Vitro Human Embryo Development
Conditions
Brief summary
The investigators suppose that adding growth factors that had been found in the female tubal fluid and uterus to In Vitro single step culture media for human embryos might improve the the embryogenesis In Vitro and the clinical outcome of the resulting off spring.
Detailed description
The IVF culture media design and upgrade underwent many ideas during the last 3 decades, we suppose that adding growth factors ( Insulin-like growth factor I, Insulin-like growth factor II, transforming growth factor-beta and epidermal growth factor ) as a promoting growth factors to Lifeglobal culture media might improve embryo development in vitro so we decide to design a trial to compare the embryogenesis by sibling Oocytes of the same patient between to arm of the same culture media and the same culture condition but with one arm with growth factors and the other is traditional one and closely monitor the outcome within the 2 arms and record the results.
Interventions
OTHERGrowth Factors and Cytokine adding to embryo culture media
OTHERNo Intervention
Nothing to Add
Sponsors
Banon IVF Center Assiut, Egypt
Ibn Sina Hospital
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
BASIC_SCIENCE
Masking
DOUBLE (Subject, Outcomes Assessor)
Eligibility
Sex/Gender
FEMALE
Age
18 Years to 40 Years
Healthy volunteers
Yes
Inclusion criteria
* all patients
Exclusion criteria
* over 40 years old
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Clinical Pregnancy rate ( % ) | 3 months |
Secondary
| Measure | Time frame |
|---|---|
| ongoing Pregnancy rate ( % ) | 6 months |
| rates of blastocyst formation and quality | 6 days of culture |
Countries
Egypt
Outcome results
None listed