Infertility
Conditions
Keywords
In Vitro culture of human embryos
Brief summary
Assessment of the embryological benefit of in vitro adding of Cytokines (LIF 5ng/ml, HB-EGF 5ng/ml and GM-CSF 2n/ml) to the culture media and closely monitor its effect on embryogenesis and implantation outcomes.
Detailed description
The IVF culture media design and upgrade underwent many ideas during the last 3 decades, we suppose that adding the Cytokines (LIF 5ng/ml, HB-EGF 5ng/ml and GM-CSF 2ng/ml) as a growth factor that have multi-ways to might improve embryo development in vitro so we decide to design a trial to compare the embryogenesis by sibling Oocytes of the same patient between two arm of the same culture media and the same culture condition. One arm with Cytokines and the other is traditional one and closely monitor the outcome within the 2 arms and record the results.
Interventions
OTHERAdding LIF, HB-EGF and GM-CSF to Lifeglobal human IVF culture media
Sponsors
Banon IVF Center Assiut, Egypt
Ibn Sina Hospital
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
OTHER
Masking
TRIPLE (Subject, Caregiver, Outcomes Assessor)
Eligibility
Sex/Gender
FEMALE
Age
18 Years to 40 Years
Healthy volunteers
Yes
Inclusion criteria
* all patients
Exclusion criteria
* over 40 years
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| ongoing pregnancy rate ( % ) | 6 months |
Secondary
| Measure | Time frame |
|---|---|
| implantation rate | 6 months |
| fertilization rate | 6 days of culture |
| clinical pregnancy rate ( % ) | 6 months |
| rates for blastocyst formation and quality | 6 days of culture |
| embryo utilization rate | 6 days of culture |
| top-quality embryo rate | 6 days of culture |
Countries
Egypt
Outcome results
None listed