Infertility
Conditions
Keywords
In Vitro, embryo quality, Intracytoplasmic sperm injection
Brief summary
Add insulin to a single step culture media for IVF embryo culture from day 0 to day 5/6 of In Vitro culture and detect the the effect upon embryo quality and implantation potential.
Detailed description
The IVF culture media design and upgrade underwent many ideas during the last 3 decades, we suppose that adding insulin as a hormon and growth factor might improve embryo development in vitro so we decide to design a trial to compare the embryogenesis by sibling Oocytes of the same patient between to arm of the same culture media and yhe same culture condition but with one arm with insulin and the other is traditional one and closely monitor the outcome within the 2 arms abd record the results
Interventions
OTHERAdding insulin to In Vitro culture media
We will add insulin for in vitro culture of human embryos
Sponsors
Banon IVF Center Assiut, Egypt
Ibn Sina Hospital
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
FEMALE
Age
18 Years to 40 Years
Healthy volunteers
Yes
Inclusion criteria
* normal responder * PCOS * Tubal factor infertility * mild asthenozoospermia
Exclusion criteria
* patient over 40
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| clinical Pregnancy rate ( % ) | 6 months |
Secondary
| Measure | Time frame |
|---|---|
| Blastocyst rate ( % ) | 6 months |
| Blastocyst quality rate ( % ) | 6 months |
Countries
Egypt
Outcome results
None listed