Oral Glucose Tolerance
Conditions
Keywords
Coffee, Chlorogenic acid, Insulin, Appetite, Metabolomics
Brief summary
Chlorogenic acid (CGA) in coffee may lower the postprandial glucose response. CGA is destroyed by dark roasting. In a controlled crossover trial, 11 healthy fasted volunteers consumed 300 mL of either light (LIR) or dark (DAR) roasted coffee, or water, followed 30 min later by a 75-g oral glucose tolerance test (OGTT). Plasma glukose and insulin, appetite, and plasma and urine metabolic profiles will be analysed. The primary aim is to investigate whether roasting affects the postprandial glucose area under the curve (AUC).
Detailed description
Epidemiologic evidence suggests that coffee consumption is associated with a lower risk of type 2 diabetes. Coffee contains caffeine and several other components that may modulate glucose regulation. The chlorogenic acids (CGA) in coffee have been indicated as constituents that may help to normalize the acute glucose response after a carbohydrate challenge. The aim of this study was to investigate whether two coffee beverages that differ in CGA content due to different roasting degrees will affect glucose regulation differently. In a controlled crossover trial, 11 healthy fasted volunteers consumed 300 mL of either light roasted (LIR) or dark roasted (DAR) coffee, or water, followed 30 min later by a 75-g oral glucose tolerance test (OGTT). Blood samples were drawn at baseline, 30, 60 and 120 min. Differences in glucose and insulin responses and insulin sensitivity index (ISI) were analyzed. The CGA and caffeine contents in the coffees were analyzed using UPLC-MS/MS.
Interventions
OTHERDAR
Volunteers ingested 300 mL of coffee with a dark roast containing low levels of chlorogenic acids followed 30 min later by by 75g glucose and 300 mL water
OTHERLIR
Volunteers ingested 300 mL of coffee with a light roast containing high levels of chlorogenic acids followed 30 min later by by 75g glucose and 300 mL water
OTHERCTR
Volunteers ingested 300 mL of water containing no chlorogenic acids followed 30 min later by by 75g glucose and 300 mL water
Sponsors
University of Copenhagen
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
PREVENTION
Masking
DOUBLE (Investigator, Outcomes Assessor)
Eligibility
Sex/Gender
ALL
Age
18 Years to 65 Years
Healthy volunteers
Yes
Inclusion criteria
Body mass index (BMI) of 18.0-30.0 kg/m2 and with no known diseases
Exclusion criteria
* systemic infections, * psychiatric or metabolic disorders, * known food allergies or intolerances related to the products used in the study (e.g. dairy or gluten), * ongoing or former drug abuse, * high intake of alcohol (defined as a weekly intake of \>7 units for women and \> 14 units for men), * pregnancy or ongoing planning of pregnancy, * vegetarianism or veganism, * participation in other scientific studies during the study period, and * blood donation during - or in the month leading up to - the study period
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Plasma glucose Area Under the Curve (Glucose AUC) | 0-120 min | The area under the plasma glucose concentration curve was calculated from 0-120min after 75g glucose was ingested. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Serum Insulin Area Under the Curve | 0-120 min | Area under the curve (AUC) for serum insulin |
| Serum insulin concentrations | 30, 60 and120 min | changes in the insulin concentration determined by a mixed model analysis and subsequently at each time point with time 0 as a co-variate. |
| Plasma glucose concentrations | 30, 60 and120 min | changes in the glucose concentration determined by a mixed model analysis and subsequently at each time point with time 0 as a co-variate. |
| Metabolic profile in plasma | 0-24 hours | Metabolic profile of plasma measured in all samples collected before the meal and postprandially from 0-120 min as well as in samples collected up to 24 hrs later. |
| Metabolic profile in urine | 0-24 hours | Metabolic profile of urine samples measured in all samples collected before the meal and postprandially from 0-120 min 120-240min as well as in samples collected up to 24 hrs later. |
| ISI(0-120) | 0-120min | Matsuda's Insulin sensitivity index |
Countries
Denmark
Outcome results
None listed