Severe Sepsis
Conditions
Brief summary
This research project is a study to immunology changes in critically ill patients with severe sepsis by using Endotoxin Activity Assay (EAA) combined with Polymyxin-B Hemoperfusion.
Interventions
PROCEDUREPolymyxin-B Hemoperfusion
Endotoxin removal
PROCEDUREStandard treatment
Sponsors
Chulalongkorn University
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
TREATMENT
Masking
DOUBLE (Subject, Caregiver)
Eligibility
Sex/Gender
ALL
Age
18 Years to 90 Years
Healthy volunteers
No
Inclusion criteria
* SIRS Criteria \> or = 2 meets definition * Source of infection \> or = 1 meet definition * Evidence of organ dysfunction \> or = 1 meet definition
Exclusion criteria
* WBC \< 5,000 /ul * Platelet \< 30,000 / ul * Pregnancy woman * Advance stage cancer patients (terminally ill) who is refuse to be resuscitated * Received blood transfusion \> 5 units in 24 hrs * Allergy to Polymyxin-B * High risk and uncontrolled bleeding * Organ transplant patients * On immunosuppressive agents within 2 weeks before study * HIV infection
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Functions of cell surface markers | 3 days | CD11b expression on PMN and HLA-DR expression on monocyte |
| Chemotaxis | 3 days | Neutrophil function |
| EAA level | 3 days | Endotoxin level |
Secondary
| Measure | Time frame |
|---|---|
| ICU length of stay | 28 days |
| Survival rate | 28 days |
| Mechanical ventilation free day | 28 days |
| Sequential Organ Failure Assessment (SOFA Score) | 28 days |
| Acute Kidney Injury and Renal Replacement Therapy incidences | 28 days |
Countries
Thailand
Outcome results
None listed