Pediatric Obesity
Conditions
Keywords
Obesity, Pediatric
Brief summary
Insulin Resistance is the best predictor of whether the obese adolescent will develop type 2 diabetes. The present studies will focus on determining what might cause fat to accumulate in the subcutaneous fat regions and lead to local inflammation, causing insulin resistance to develop in obese adolescents.
Detailed description
The purpose of this study is to test whether the reduced transcription of key lipogenic/adipogenic genes in abdominal subcutaneous adipose tissue (SAT) in obese adolescents with a High VAT/SAT (visceral adipose tissue/subcutaneous adipose tissue) ratio translates functionally into a reduced in vivo triglyceride (TG) synthesis and adipocyte proliferation, which in turn will contribute to ectopic fat accumulation and insulin resistance (IR). To test whether the storage capacity of gluteal SAT is linked to the level of VAT/SAT fat distribution in obese adolescents. To explore if changes in dietary fat intake (palmitate intake) alters ceramide concentration in both plasma and adipose tissue (AT) and the Inflammasome complex in the different fat distribution phenotypes and their associations with IR.
Interventions
OTHERPalmitate Diet
Subject with high VAT/SAT ratio will be randomized to a high or low palmitate oil diet.
Sponsors
Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD)
Yale University
Study design
Observational model
COHORT
Time perspective
CROSS_SECTIONAL
Eligibility
Sex/Gender
ALL
Age
12 Years to 18 Years
Healthy volunteers
Yes
Inclusion criteria
* Overweight (85-95th% age and gender specific) or Obese (\>95th% age and gender specific) * Ages 12-18 years * Absence of any endocrinopathy * Absence of any therapy with medication known to alter glucose metabolism (i.e.: Oral steroids, certain psychiatric medications such as Xeleca, Lithium, Paxil) * English Speaking subjects
Exclusion criteria
* Any disease known to alter glucose metabolism (T2DM) * Diuretic Medication * Pregnant or breastfeeding * Type 1 or 2 diabetes
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Fat Biopsies | Baseline | 1\) To test whether the reduced transcription of key lipogenic/adipogenic genes in abd SAT in obese adolescents with a High VAT/SAT ratio translates functionally into a reduced in vivo TG synthesis and adipocyte proliferation, which in turn will contribute to ectopic fat accumulation and IR. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| MRI | Baseline | Visceral to Subcutaneous fat storage ratio |
Countries
United States
Outcome results
None listed