Heart Failure, Obesity
Conditions
Keywords
obese cardiomyopathy, mitochondria, contractile dysfunction
Brief summary
The investigators aim to explore obese cardiomyopathy by studying contractile twitch force, sarcomere sensitivity to calcium and mitochondrial function in atrial myocardial samples of patients grouped according to their body mass index, i.e. normal weight, overweight and obese.
Detailed description
Obesity is associated with the development of heart failure, regardless of coronary artery disease or other comorbidities. Right atrial samples obtained from patients undergoing cardiac surgery and presenting no sign of clinical cardiomyopathy will be grouped according to patient's body mass index. Atrial trabeculae will be explored to dissect out the respective contribution of (i) sarcoplasmic reticulum dysfunction (isometric twitch force contraction analysis), (ii) abnormal sarcomere sensitivity to calcium assessed in permeabilized fibers and (iii) mitochondrial dysfunction to intrinsec contractile dysfunction of myocardium in obese patients.
Interventions
OTHERfunctional tissu atrial analysis
Sponsors
University Hospital, Lille
Study design
Observational model
CASE_CONTROL
Time perspective
CROSS_SECTIONAL
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
No
Inclusion criteria
* patient schedulled for coronary artery bypass graft surgery or aortic valve replacement surgery with extracorporeal circulation * must be over 18 years of age * must be able to read and understand the consent form in French * must be in sinus rhythm at inclusion time
Exclusion criteria
* medical history of type 1 diabetes * medical history of sustained atrial arrhythmia * pregnancy * class III anti-arhythmic drug
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Intrinsic myocardial contractile force | the day of cardiac surgery | twitch force developed by atrial trabeculae assessed in isometric conditions in atrial samples |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| sarcomere sensitivity to calcium | the day of cardiac surgery | force-calcium concentration relation assessed on permeabilized atrial fibers |
| Mitochondrial function | the day of cardiac surgery | assesment of mitochondrial respiration and calcium retention capacity by oxygraphic measurement in saponin-permeabilized atrial fibers |
Countries
France
Outcome results
None listed