Large-scale Expansion and Characterization of Mesenchymal Stem Cells for Clinical Application

Large-scale ex Vivo Expansion and Characterization of Synovial Membrane Mesenchymal Stem Cells (SM-MSC) and Infrapatellar Fat Pad Mesenchymal Stem Cells (IF-MSC) for Clinical Application

Status

UNKNOWN

Phases

Unknown

Study type

Observational

Source

ClinicalTrials.gov

Registry ID

NCT02375568

Enrollment

Registered

2015-03-02

Start date

2014-05-31

Completion date

2015-08-31

Last updated

2015-03-02

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Knee Injuries

Keywords

mesenchymal stem cells

Brief summary

According to many recent research, mesenchymal stem cells (MSC) isolated from synovial membrane and infrapatellar fat pad have higher ex vivo proliferation capacity, multipotency and ability to undergo chondrogenesis. Based on this concept, the investigators plan to get two removed tissues (synovial membrane and infrapatellar fat pad) from patients who have an operation of total knee replacement. The investigators are going to isolate and expand MSC from these two tissues respectively, and then compare the chondrogenesis ability for clinical application. In the other hand, the investigators plan to build techniques and procedures which comply with Good Tissue Practices (GTP)

Interventions

OTHER103024-F

mesenchymal stem cell isolation

Study design

Observational model

CASE_ONLY

Time perspective

PROSPECTIVE

Eligibility

Sex/Gender

ALL

Healthy volunteers

Inclusion criteria

Patient who have an total knee replacement surgery

Exclusion criteria

Patient who don't sign the agreement

Design outcomes

Primary

Measure	Time frame
Cell viability ratio of synovial membrane MSC and infrapatellar fat pad MSC from the first to the tenth cell passage	6 months
Doubling time of synovial membrane MSC and infrapatellar fat pad MSC from the first to the tenth cell passage	6 months
Percentage expression of cluster differentiation 90,105, 34, 45 surface markers of synovial membrane MSC and infrapatellar fat pad MSC from the first to the tenth cell passage	6 months

Secondary

Measure	Time frame
Alcian blue staining of synovial membrane MSC and infrapatellar fat pad MSC	6 months
Tyep II collagen and aggrecan gene expression of synovial membrane MSC and infrapatellar fat pad MSC	6 months

Countries

Taiwan

Contacts

Primary ContactChih-Hung Chang, MD, Ph.D

orthocch@mail.femh.org.tw886-2-89667000

Outcome results

None listed

Source: ClinicalTrials.gov · Data processed: Feb 4, 2026