Periimplantitis, Dental Plaque, Periodontal Diseases, Caries, Oral Surgical Wound Infection
Conditions
Keywords
anti-infective agents, local/therapeutic use, chlorhexidine, dental desinfectants, polyhexamethylene biguanide, mouthrinse
Brief summary
This is a three arm randomized trial. The aim is to evaluate the antimicrobial activity of chlorhexidine and polyhexamethylene biguanide oral antiseptics on the microorganisms of the oral cavity. A total of 30 healthy volunteers will be enrolled and randomly allocated to control group (CG, n=10), which will be instructed to rinse the mouth with 10ml of a sterile saline solution for one minute, to chlorhexidine group (ClG, n=10), which will be instructed to rinse the mouth with 10ml of an 0.12% chlorhexidine solution, for one minute, or to polyhexamethylene biguanide group (PG, n=10), which will be instructed to rinse the mouth with 10ml of an 0.07% polyhexamethylene biguanide solution, for one minute. Samples of saliva will be collected before the mouth wash and after 30, 60 and 180 minutes. Samples will be plated on manitol agar, mitis salivarius agar, EMB agar and Sabouraud agar. Samples will be processed by a blinded microbiologist.
Interventions
DRUGchlorhexidine
The volunteers will be instructed to mouthwash with 10ml of a sterile saline solution for 30 seconds and than make the mouthwash with 10ml of an 0.12% chlorhexidine solution. Samples of saliva will be collected immediately before the mouth wash and after 30, 60 and 180 minutes.
The volunteers will be instructed to mouthwash with 10ml of a sterile saline solution for 30 seconds and than make the mouthwash with 10ml of an 0,07% solution Polyhexamethylene biguanide. Samples of saliva will be collected before the mouth wash and after 30, 60 and 180 minutes.
The volunteers will be instructed to mouthwash with 10ml of a sterile saline solution for 30 seconds and than make the mouthwash with 10ml of a sterile saline solution. Samples of saliva will be collected before the mouth wash and after 30, 60 and 180 minutes.
Sponsors
Universidade do Vale do Sapucai
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
PREVENTION
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
18 Years to 30 Years
Healthy volunteers
Yes
Inclusion criteria
* patients aged 18-30 years
Exclusion criteria
* patients with a hypersensitivity or allergic response by some component of the formula antiseptics, * pregnant women, * lactating, * history of periodontal disease, * smoking, * decompensated diabetics, * patients undergoing chemotherapy, * radiotherapy patient, * transplanted patient and refuses to participate, * not signing a term of free and informed concentiment
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Number of Colony Forming Units | 180 minutes | Samples of saliva will be collected before the mouth wash and after 30, 60 and 180 minutes. |
Outcome results
None listed