Extracellular Matrix Alteration, HEMORRHOIDS
Conditions
Keywords
Hemorrhoids, MMP, NGAL, Matrix Metalloproteinases
Brief summary
An association between hemorrhoidal disease and matrix metalloproteinases (MMPs) has been described previously. MMPs regulate extracellular structural proteins and tissue remodeling. Neutrophil gelatinase-associated lipocalin (NGAL) is involved in the regulation of MMP activity. The aim of this work was to study the relationship between the levels of MMPs and NGAL and different stages hemorrhoids. The study provides potentially important insights to the understanding of the natural history of Hemorrhoids and MMPs and NGAL regulation.
Detailed description
Introduction. An association between hemorrhoidal disease and matrix metalloproteinases (MMPs) has been described previously. MMPs regulate extracellular structural proteins and tissue remodeling. Neutrophil gelatinase-associated lipocalin (NGAL) is involved in the regulation of MMP activity. The aim of this work was to study the relationship between the levels of MMPs and NGAL and different stages hemorrhoids. Methods. In a multicenter, open-label, prospective study, patients with hemorrhoids were recruited and represented the Group I (patients with symptomatic hemorrhoids - Grade I to IV sec. Goligher), a further group of healthy volunteer subjects were also recruited as the control group (Group II). Enzyme-linked immunosorbent assay and Western blot analysis will evaluate the levels of immunoreactive MMPs and NGAL in all patients with hemorrhoids.
Interventions
PROCEDUREHemorrhoidectomy
Grade III and IV patients were treated with surgery according to Milligan-Morgan (Milligan-Morgan laser optical fibers variant) procedure. All patients will perform blood sampling for MMPs and NGAL plasma levels evaluation
OTHERblood sampling
blood sampling for MMPs and NGAL plasma levels evaluation (ELISA test)
OTHERtissue sampling
tissue sampling for MMPs and NGAL tissue levels evaluation (Western Blot)
Sponsors
University of Catanzaro
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
18 Years to 70 Years
Healthy volunteers
Yes
Inclusion criteria
* Patients with hemorrhoids of both sex
Exclusion criteria
* Patients with cancer * Hepatic failure * Infectious or autoimmune diseases * Arthritis * Arterial aneurysms * Hernias * Chronic venous disease * Nephritis, vascular ulcers, fibrosis, and other diseases associated with increased levels of MMPs were excluded from the study. * Patients treated with corticosteroids or cytostatic drugs were also excluded.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| MMPs and NGAL plasma and tissue levels measurement in patients with hemorrhoids | 15 months | A new insight in the understanding of the natural history of. MMPs and NGAL play a role in development of disease and may represent molecular markers for the prevention of hemorrhoidal thrombosis. Unit of Measure: MMPs and NGAL plasma levels will be expressed as ng/mL. Tissue expression of MMPs and NGAL will be expressed as Arbitrary Units |
Outcome results
None listed