Overweight, Obesity
Conditions
Brief summary
The potential health effects of high intake of lean or fatty fish will be investigated in overweight or obese adults. Participants consume 750g/week of fillets of fish for 8 weeks. Hypothesis: High intake of fatty or lean fish will beneficially affect glucose regulation and the immune system.
Interventions
OTHERLean fish
OTHERFatty fish
Sponsors
Bergen Medical Research Foundation
Skretting ASA
Leroy Seafood Group ASA
University of Bergen
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
TREATMENT
Masking
SINGLE (Outcomes Assessor)
Eligibility
Sex/Gender
ALL
Age
18 Years to 69 Years
Healthy volunteers
Yes
Inclusion criteria
* BMI ≥27 kg/m2 * fasting blood glucose ≤7.0 mmol/L
Exclusion criteria
* pregnancy * incompatibility with fish consumption (allergies, intolerance and/or dislike) * diagnosed diabetes mellitus, heart disease or gastrointestinal diseases * use of medications affecting lipid metabolism or glucose homeostasis * use of anti-inflammatory medications * use of supplements containing long chain n-3 fatty acids * intentional weight loss * and large fluctuation in body weight (\>3 kg) over the previous two months
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Changes in serum concentrations of glucose | baseline and 8 weeks |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Changes in serum vitamin concentrations after fish intake. | baseline and 8 weeks | Serum concentrations of fat-soluble and water-soluble vitamins were measured, and dietary intakes of vitamins were estimated based on reported food intake from 5-day food diaries. |
| Changes in serum and urine concentrations of amino acids and metabolites of amino acids after fish intake. | baseline and 8 weeks | Concentrations of amino acids and amino acid metabolites were measured in fasting serum and morning urine |
| Changes in fecal microbiota profile after fish intake. | baseline and 8 weeks | 54 DNA bacterial markers targeting more than 300 bacteria based on their 16S rRNA sequence in seven variable regions were analysed in feces (72h collection) |
| Changes in fecal and serum concentrations of lipids and bile acids after fish intake. | baseline and 8 weeks | Fat, cholesterol and total bile acids were quantified in feces (72h collection) and fasting serum. |
| Changes in markers of oxidative stress after fish intake. | baseline and 8 weeks | Antioxidant enzymes, antioxidant enzyme cofactors and biomarkers of oxidative stress were quantified in serum |
| Changes in insulin, insulin C-peptide, leukocyte fatty acids, leukocyte function, body weight and composition, and intake of energy and macronutrients within the groups over time. | baseline and 8 weeks | — |
| Quantification of markers of kidney dysfunction | baseline and 8 weeks | Urine concentrations of cystatin C and T-cell immunoglobulin mucin-1 (relative to creatinine) were measured as markers of kidney function |
| Changes in amino acids and metabolites of amino acids after a standardized meal. | baseline | Concentrations of amino acids and amino acid metabolites were measured in fasting and postprandial serum |
| Changes in metabolites and co-factors involved in one-carbon metabolism after a standardized meal | baseline | Concentrations of relevant metabolites and co-factors were measured in fasting and postprandial serum |
| Comparisons of amino acid concentrations in serum and urine between men and women | baseline | Concentrations of amino acids and amino acid metabolites were measured in serum and urine |
| Comparisons of metabolites and co-factors involved in one-carbon metabolism in serum and urine between men and women | baseline | Concentrations of relevant metabolites and co-factors were measured in serum and urine |
| Changes in total neopterin after fish intake | baseline and 8 weeks | Total neopterin was measured in serum. |
Countries
Norway
Outcome results
None listed