Focus is Understanding Absorption of Garlic Metabolites
Conditions
Brief summary
The study will consist of a single 5-day dietary treatment period. Subjects will consume an Allium-free diet for 3 days. Alliums are vegetables such as garlic, onions, chives, shallots, and leeks. On day 4, subjects will consume a breakfast consisting of garlic and will then provide blood and urine samples for 10 hours, and on the following morning.
Interventions
OTHERGarlic Intervention
Participants will consume an Allium-free controlled diet for 3 days. On the 4th day, they will consume a test meal containing garlic followed by blood and urine collections for 10 hours. On the 5th day, participants will have a fasting blood and urine collection.
Sponsors
USDA Beltsville Human Nutrition Research Center
Study design
Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
25 Years to 75 Years
Healthy volunteers
Yes
Inclusion criteria
* Men and women of age 25 to 75 years at beginning of study * BMI between 18.5 and 30 kg/m2 * Fasting glucose \< 126 mg/dl * Blood pressure \< 160/100 (controlled with certain medications) * Fasting total blood cholesterol \< 280 mg/dl * Fasting triglycerides \< 300 mg/dl
Exclusion criteria
* Younger than 25 years old or older than 75 years old * Use of blood-thinning medications such as Coumadin (warfarin), Dicumarol (dicumarol), or Miradon (anisindione) * Presence of kidney disease, liver disease, gout, certain cancers, thyroid disease, gastrointestinal, other metabolic diseases or malabsorption syndromes. * Have been pregnant during the previous 12 months, are currently pregnant or lactating, or plan to become pregnant during the study * Type 2 diabetes requiring the use of oral antidiabetic agents or insulin * History of eating disorders or other dietary patterns which are not consistent with the dietary intervention (e.g., vegetarians, very low fat diets, high protein diets) * Use of prescription or over-the-counter antiobesity medications or supplements (e.g., phenylpropanolamine, ephedrine, caffeine) during and for at least 6 months prior to the start of the study or a history of a surgical intervention for obesity * Active cardiovascular disease (such as a heart attack or procedure within the past three months or participation in a cardiac rehabilitation program within the last three months, stroke, or history/treatment for transient ischemic attacks in the past three months, or documented history of pulmonary embolus in the past six months). * Use of any tobacco products in past 6 months * Unwillingness to abstain from herbal supplements for two weeks prior to the study and during the study * Known (self-reported) allergy or adverse reaction to garlic * Inability to tolerate garlic * Unable or unwilling to give informed consent or communicate with study staff * Self-report of alcohol or substance abuse within the past twelve months and/or current acute treatment or rehabilitation program for these problems (Long-term participation in Alcoholics Anonymous is not an exclusion) * Other medical, psychiatric, or behavioral factors that in the judgment of the Principal Investigator may interfere with study participation or the ability to follow the intervention protocol
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Allyl methyl sulfide | 24 hour | Metabolite will be analyzed in blood and urine. |
| N-acetyl allylcysteine sulfoxide | 24 hour | Metabolite will be analyzed in blood and urine. |
| N-acetyl-S-trans-1-propenylcysteine sulfoxide | 24 hour | Metabolite will be analyzed in blood and urine. |
Countries
United States
Outcome results
None listed