Infertility
Conditions
Keywords
single-step media, embryo development, blastocyst formation, embryo utilization
Brief summary
This prospective study with sibling oocytes will evaluate the efficiency of two commercial single-step media, namely Sage 1-step and Continuous Single Culture Medium (CSCM), in terms of embryo utilization rates, blastocyst formation rates, embryo quality and pregnancy rates. Embryos will be cultured continuously for up to 6 days under identical conditions.
Interventions
OTHERembryo culture in different single-step media
Half of each patient's oocytes will be randomly allocated to be cultured either in Sage 1-step medium (Origio) or in CSC medium (Irvine Scientific) for up to six days following oocyte retrieval.
Sponsors
Eugonia
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
TREATMENT
Masking
QUADRUPLE (Subject, Caregiver, Investigator, Outcomes Assessor)
Eligibility
Sex/Gender
FEMALE
Age
18 Years to 40 Years
Healthy volunteers
Yes
Inclusion criteria
* at least 10 oocytes retrieved
Exclusion criteria
* frozen oocytes * testicular biopsy * less than 10 oocytes retrieved * older than 40 years
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Utilization rates | 6 days post oocyte retrieval | Utilization = embryos selected for transfer and cryopreservation |
| Blastocyst formation | 5 days post oocyte retrieval | Number of blastocysts on day 5 post oocyte retrieval per fertilized oocytes |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Embryo quality on day 3 | 3 days post oocyte retrieval | — |
| blastocyst quality on day 5 | 5 days post oocyte retrieval | — |
| positive pregnancy test | 15 days post oocyte retrieval | — |
| clinical pregnancy | 7 weeks of gestation | presence of gestational sac with positive fetal heartbeat |
Countries
Greece
Outcome results
None listed