Oxidative Stress
Conditions
Keywords
Antioxidants, Protein Carbonylation, Malondialdehyde, Punicaceae, Athletic Performance
Brief summary
The purpose of this study is to evaluate modifications of plasma protein carbonyls and malondialdehyde levels in endurance-based athletes after three weeks of pomegranate juice consumption.
Interventions
DIETARY_SUPPLEMENTPomegranate juice
Intake of 200 ml of pomegranate juice or pomegranate juice dilution during 21 days after exercise.
Sponsors
Vitalgrana Pomegranate SL
Universidad Miguel Hernandez de Elche
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
TREATMENT
Masking
TRIPLE (Subject, Investigator, Outcomes Assessor)
Eligibility
Sex/Gender
MALE
Age
18 Years to 50 Years
Healthy volunteers
Yes
Inclusion criteria
* Perform specific endurance training sessions * More than 1 hour per session and more than 3 sessions per week * Have participated recently in a half marathon or similar event
Exclusion criteria
* Intake of antioxidant or anti-inflammatory supplementation * Present a chronic disorder * Non-smoker * No-alcoholic beverage consumption
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Protein carbonyls levels changes in plasma | day 0 and 22 | Protein carbonyl derivatives were determined using an adaptation of the colorimetric method published in Levine et al. (1994) based in the formation of a stable 2,4-dinitrophenyl (DNP) hydrazone product after the addition of 2,4-dinitrophenylhydrazine (DNPH) to tha plasma. Protein carbonyls were calculated as nmoles·mg-1 protein |
| Malondialdehyde levels changes in plasma | day 0 and 22 | Malondialdehyde was determined by high pressure liquid chromatography (HPLC) with fluorescence detection according to the method described by Laporta et al. (2007). Malondialdehyde levels were calculated as nmoles·g-1 protein |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Blood biochemical parameters changes in plasma | day 0 and 22 | Blood biochemical parameters is a composite outcome measure consisting in circulating plasma glucose (mg·dL-1), HDL-cholesterol (mg·dL-1), triglycerides (mg·dL-1), ferritin (ng·mL-1), lactate (mg·dL-1), sodium (mEq·L-1), potassium (mEq·L-1), creatine kinase (U·L-1), aspartate aminotransferase (U·L-1) and alanine aminotransferase (U·L-1) were analyzed through biochemistry methods. |
| C-reactive protein levels changes in plasma | day 0 and 22 | Determination by immunoassay of C-reactive protein (ng·mL-1) levels in plasma. |
| Soluble E-selectin levels changes in plasma | day 0 and 22 | Determination by immunoassay of soluble E-selectin (ng·mL-1) levels in plasma. |
Outcome results
None listed