Healthy
Conditions
Keywords
volunteers
Brief summary
Evaluate if gene expression changes in SOD2 explain the health benefits of high polyphenols cocoa
Detailed description
Evaluate if gene expression changes in SOD2 explain the health benefits of high polyphenols cocoa.
Interventions
OTHERCocoa Pure
Cocoa Pure.
OTHERPlacebo
Placebo.
Sponsors
Société des Produits Nestlé (SPN)
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
DOUBLE (Subject, Investigator)
Eligibility
Sex/Gender
ALL
Age
18 Years to 40 Years
Healthy volunteers
Yes
Inclusion criteria
* Healthy adults * BMI between 18.5 and 30 kg/m2 * Sedentary to moderate physical activity habits
Exclusion criteria
* Consumption of dietary supplements, antioxidants or anti-inflammatory medication, such as aspirin, ibuprofen, naproxen, or any medication that can affect the study outcomes * Actively smoking * Suspected abuse of alcohol or illicit drugs * Significant illness within two weeks prior to study start or any active systemic infection or medical condition that may require treatment or therapeutic intervention during the study * Subject who cannot be expected to comply with study procedures * Currently participating or having participated in another clinical trial during last 4 weeks prior to the beginning of this study * Consumption of chocolate or its derivates 24 hours before the blood sample taken
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Measurement of changes in SOD2 gene expression | after acute dose and 4 weeks of chronic dose |
Secondary
| Measure | Time frame |
|---|---|
| Determination of DNA methylation pattern in the chronic phase of the study | After 4 weeks of chronic dose |
| Evaluation of (-)epicatechin bioavailability in plasma | After 4 weeks of chronic dose |
| Determination of plasma antioxidant capacity as a marker of oxidative stress | After 4 weeks of chronic dose |
Outcome results
None listed