Oxidative Stress
Conditions
Keywords
Endogenous antioxidants, Antioxidant supplements, Vitamin Supplements, Exercise adaptations, Oxidative stress, Redox regulation, Cycling - exercise
Brief summary
This study will investigate whether increased habitual intake of antioxidant supplements effects the concentration of endogenous antioxidants, the response of antioxidants to exercise, and differences in oxidative damage compared to a non-supplemented group.
Interventions
None listed
Sponsors
University of Birmingham
Study design
Observational model
CASE_CONTROL
Time perspective
OTHER
Eligibility
Sex/Gender
MALE
Age
18 Years to 35 Years
Healthy volunteers
Yes
Inclusion criteria
* Male, * BMI 18.8 29.9 kg/m2 * cycle 3-7 times a week for durations longer than 60 minutes * cycled for a minimum of 3 years * accustomed to exhaustive cycling protocols * healthy (no known cardiovascular or metabolic disease)
Exclusion criteria
* answering NO to any question on the general health questionnaire would result in exclusion from the study * current participation in another clinical study * current or recent smoker (last 30 days) * prescription or non-prescription of anti-inflammatory use \>30 days prior to the experiment commencing or any other medications that in the opinion of the investigator may interfere with redox signalling
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Difference in Resting Levels of Endogenous Antioxidant Proteins in Skeletal Muscle - Superoxide Dismutase 2 (SOD-2) | Protein concentrations will be measured at baseline only | To examine the effects of habitual vitamin and antioxidant supplementation on endogenous antioxidant production, participants will have a muscle biopsy taken at rest to examine any differences in endogenous antioxidant protein concentrations |
Countries
United Kingdom
Participant flow
Participants by arm
| Arm | Count |
|---|---|
| Supplemented The group consists of well-trained cyclists who habitually consume vitamin/antioxidant supplements for a period longer than 6 months | 4 |
| Non-Supplemented The group consists of well-trained cyclists who do not consume vitamin/antioxidant supplements | 10 |
| Total | 14 |
Baseline characteristics
| Characteristic | Supplemented | Non-Supplemented | Total |
|---|---|---|---|
| Age, Categorical <=18 years | 0 Participants | 0 Participants | 0 Participants |
| Age, Categorical >=65 years | 0 Participants | 0 Participants | 0 Participants |
| Age, Categorical Between 18 and 65 years | 4 Participants | 10 Participants | 14 Participants |
| Region of Enrollment United Kingdom | 4 participants | 10 participants | 14 participants |
| Sex: Female, Male Female | 0 Participants | 0 Participants | 0 Participants |
| Sex: Female, Male Male | 4 Participants | 10 Participants | 14 Participants |
Adverse events
| Event type | EG000 affected / at risk | EG001 affected / at risk |
|---|---|---|
| deaths Total, all-cause mortality | — / — | — / — |
| other Total, other adverse events | 0 / 4 | 0 / 10 |
| serious Total, serious adverse events | 0 / 4 | 0 / 10 |
Outcome results
Primary
Difference in Resting Levels of Endogenous Antioxidant Proteins in Skeletal Muscle - Superoxide Dismutase 2 (SOD-2)
To examine the effects of habitual vitamin and antioxidant supplementation on endogenous antioxidant production, participants will have a muscle biopsy taken at rest to examine any differences in endogenous antioxidant protein concentrations
Time frame: Protein concentrations will be measured at baseline only
| Arm | Measure | Value (MEAN) | Dispersion |
|---|---|---|---|
| Supplemented | Difference in Resting Levels of Endogenous Antioxidant Proteins in Skeletal Muscle - Superoxide Dismutase 2 (SOD-2) | 0.42 Arbitrary Units | Standard Deviation 0.2 |
| Non-Supplemented | Difference in Resting Levels of Endogenous Antioxidant Proteins in Skeletal Muscle - Superoxide Dismutase 2 (SOD-2) | 0.68 Arbitrary Units | Standard Deviation 0.32 |