Nitrate Supplementation; Source

The Effect of Nitrate Supplementation: Source of Supplementation

Status

Completed

Phases

Phase 4

Study type

Interventional

Source

ClinicalTrials.gov

Registry ID

NCT02271633

Acronym

NO-what

Enrollment

Registered

2014-10-22

Start date

2014-09-30

Completion date

2016-03-31

Last updated

2016-03-16

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Blood Pressure, Nutritional Physiological Phenomena

Keywords

Nitrate, Bioavailability, Sports

Brief summary

The main aim of the current study will be to compare the effects of ingesting different nitrate-rich sources on plasma nitrite concentrations.

Detailed description

Oral ingestion of nitrate (NO3-) has been shown to increase plasma nitrate and nitrite levels. Significant lowering of pulmonary oxygen uptake during exercise, as well as significantly lower blood pressure values at rest have been observed after supplementation with nitrate. However, no study has yet described a direct comparison between different nitrate-rich beverages with respect to the bioavailability of nitrate/nitrite and the possible hemodynamic effects. Based on the gaps in current literature, our main goal will therefore be to gain further insight into the effects of nitrate supplementation with different sources on plasma nitrite levels.

Interventions

DIETARY_SUPPLEMENTSodium nitrate

DIETARY_SUPPLEMENTBeetroot juice

DIETARY_SUPPLEMENTSpinach

DIETARY_SUPPLEMENTRocket salad

Study design

Allocation

RANDOMIZED

Intervention model

CROSSOVER

Primary purpose

BASIC_SCIENCE

Masking

NONE

Eligibility

Sex/Gender

ALL

Age

18 Years to 45 Years

Healthy volunteers

Yes

Inclusion criteria

* 18.5 \< BMI \< 30 kg/m2 * Engagement in regular physical activity ( \> 1 h a week)

Exclusion criteria

* Use of medication * Smoking * Currently supplementing diet with nitrate * Lactose intolerance

Design outcomes

Primary

Measure	Time frame	Description
Plasma nitrite levels	Baseline until 5 hours after ingestion	Plasma nitrite levels following ingestion of dietary nitrate

Secondary

Measure	Time frame	Description
Tongue scraping to assess bacterial mouth flora.	5 h post-ingestion	Tongue scraping to assess bacterial mouth flora.
Plasma levels of nitrate	Baseline until 5 hours post-ingestion	Plasma nitrate levels following ingestion of dietary nitrate
Resting blood pressure	Baseline, 2.5 hours and 5 hours post-ingestion	Resting blood pressure measures following ingestion of dietary nitrate

Other

Measure	Time frame	Description
Baseline characteristics	1 hour during visit 1 (Screening)	Resting blood pressure, height, body weight, age, BMI

Countries

Netherlands

Outcome results

None listed

Source: ClinicalTrials.gov · Data processed: Mar 13, 2026