Coronary Artery Disease, Endothelial NO-synthase
Conditions
Keywords
coronary artery disease, acute coronary syndrome, angina pectoris, non-ST-segment elevation myocardial infarction, red blood cells, erythrocytes
Brief summary
The aim of this study is measurement of expression and activity of RBC eNOS in different clinical cohorts of patients with coronary artery disease and acute coronary syndrome and to investigate the importance of the NO-stimulated reaction cascade in terms of sGC and PKG.
Interventions
None listed
Sponsors
Heinrich-Heine University, Duesseldorf
Study design
Observational model
COHORT
Time perspective
OTHER
Eligibility
Sex/Gender
ALL
Age
40 Years to 80 Years
Healthy volunteers
Yes
Inclusion criteria
* informed consent * for ACS(acute coronary syndrome)-Group: angiographic coronary artery disease; acute coronary syndrome, defined by EITHER unstable angina pectoris with significant coronary stenosis (troponin negative, without significant ST-elevation) OR low-risk NSTEMI (Troponin positive, without significant ST-elevation) * for stable CAD-Group: angiographic coronary artery disease; no acute coronary syndrome within the last 3 months * healthy control group: No clinical or angiographical signs of apparent atherosclerosis
Exclusion criteria
* acute inflammation (CRP \> 1 mg/dl, leukocyte \> 11.000/µl) * malignant diseases * pregnancy * medication with NO-donors ( e.g.isosorbide mononitrate, isosorbide dinitrate, glyceryl trinitrate, Molsidomin, Ranexa) * patients with STEMI or high-risk NSTEMI
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Determination of red cell endothelial nitric oxide-synthase (eNOS)-expression and - activity | baseline, 3 hours and 3 month |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Electrocardiogram | baseline, 3 hours and 3 month | — |
| Deformability and stability of red blood cell membrane | baseline, 3 hours and 3 month | by Laser assisted Optical Rotational Cell Analyzer (LORCA) |
| Microvascular function | baseline, 3 hours and 3 month | Determination of microvascular function with Laser-Doppler Perfusion Imaging |
| Peripheral and central hemodynamic measurement | baseline, 3 hours and 3 month | — |
| Determination of arterial stiffness | baseline, 3 hours and 3 month | Determination of arterial stiffness with pulse wave analysis during blood pressure measurement or echocardiography. |
| determination of flow-mediated dilation | baseline, 3 hours and 3 month | — |
| Determination of nitrate and nitrite in blood plasma and red blood cells (RBC) | baseline, 3 hours and 3 month | — |
| Determination of nitric oxide and cyclic guanosine monophosphate (cGMP) in blood plasma and in red blood cells | baseline, 3 hours and 3 month | — |
| Changes of tetrahydrobiopterin-metabolism | baseline, 3 hours and 3 month | — |
| Aging and turnover of red blood cells | baseline, 3 hours and 3 month | — |
| Redox status of red blood cells | baseline, 3 hours and 3 month | Determination of redox status of red blood cells will be measured on free thiole with thioltracker and reactive oxygen species( ROS) by dihydrodichlorofluorescein |
| Aggregation of red blood cells | baseline, 3 hours and 3 month | Determination of aggregation of red blood cells with LORCA |
| Platelet aggregometry | baseline, 3 hours and 3 month | — |
| Expression and function of soluble guanylyl cyclase (sGC) in red blood cells | baseline, 3 hours and 3 month | measurement of enzyme expression and activity |
| Expression and activity of Protein Kinase G (PKG) | baseline, 3 hours and 3 month | measurement of enzyme expression and activity |
| Determination of leucocyte populations and leucocyte function | baseline, 3 hours and 3 month | flow-cytometry |
| Determination of endothelial microparticles | baseline, 3 hours and 3 month | — |
Countries
Germany
Outcome results
None listed