Red Blood Cells as a Reflection of Endothelial Function in Old Age

Status

Completed

Phases

Unknown

Study type

Observational

Source

ClinicalTrials.gov

Registry ID

NCT02265003

Enrollment

140

Registered

2014-10-15

Start date

2012-07-31

Completion date

2020-12-31

Last updated

2021-02-16

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Endothelial Function, Nitric Oxide Pool in Red Blood Cells and Blood Plasma, Red Blood Cells

Keywords

red blood cells, endothelial function, nitric oxide pool in red blood cells, nitric oxide poll in blood plasma, nitric oxide synthase III - expression, oxidative status, nitric oxide signalling

Brief summary

The aim of this study is to find out, how far red blood cells reflect age-depended changes in endothelial function.

Detailed description

It will be investigated with determination of endothelial function (measured as flow-mediated vasodilation) and of circulated nitric oxid-pool in red blood cells and in blood plasma, how far red blood cells reflect age-depended changes in endothelial function.

Interventions

None listed

Study design

Observational model

COHORT

Time perspective

PROSPECTIVE

Eligibility

Sex/Gender

ALL

Age

18 Years to No maximum

Healthy volunteers

Yes

Inclusion criteria

Groups 1-3: Inclusion Criteria: * healthy subjects * informed consent

Exclusion criteria

* hypertension (blood pressure ≥140/90 mmHg), * hypercholesterolemia (LDL ≥250 mg/dl, HDL ≤45 mg/dl), * diabetes mellitus (fasting plasma glucose ≥128 mg/dl) * acute inflammation (CRP \>0.5 mg/dl) * malignant diseases * heart failure (NYHA III-IV) * arrhythmia of high degree * acute or terminated kidney disease * cardiovascular diseases ( cardiac artery disease, peripheral vascular disease and cerebrovascular diseases) * anemia * pregnancy Group 4 : Inclusion Criteria: * angiographical signs of apparent atherosclerosis (cardiac artery disease or peripheral vascular disease or degenerative valvular heart disease) * informed consent

Design outcomes

Primary

Measure	Time frame	Description
Determination of endothelial function	baseline	measured as flow-mediated vasodilation

Secondary

Measure	Time frame	Description
Determination of nitric oxide(NO)-pool in red blood cells and blood plasma	baseline	—
Determination of erythrocytes parameter	baseline	It will be determinated: oxidative status, signalling of nitric oxide, function, creation, amount and aging of red blood cells, cell-to-cell-interaction, microRNA and inflammation
Cardiovascular Parameter	baseline	It will be determinated: hemodynamic, microcirculation and physicomechanic

Countries

Germany

Outcome results