Obesity
Conditions
Keywords
bacterial, gut, type 2 diabetes., 16S rDNA gene, intestinal crypt
Brief summary
The investigators will compare 16sRDNA profile in cases and controls in order to identify bacteria associated with metabolic diseases that reside both in intestinal crypts and in blood.
Detailed description
Experimental evidence suggests that bacterial translocation from gut to tissue could be an initial step leading to diabetes. To test this hypothesis, a bacterial component that is highly conserved between different species of bacteria, the 16S rDNA gene will be analysed both in intestinal crypt and in blood in patients with type 2 diabetes, obese patients and control.
Interventions
OTHERpatients free type 2 diabetes
collection of blood sampling and biopsies
OTHERobese patients
collection of blood sampling and biopsies
OTHERdiabetic patients
collection of blood sampling and biopsies
OTHERpatients free of obesity and diabetes
collection of blood sampling and biopsies
Sponsors
University Hospital, Toulouse
Study design
Allocation
NON_RANDOMIZED
Intervention model
PARALLEL
Primary purpose
DIAGNOSTIC
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
35 Years to 75 Years
Healthy volunteers
Yes
Inclusion criteria
* Between 35 and 75 years old * Colonoscopy and biopsies required Diabetes population: * Type 2 diabetes Obese population: * Android obesity
Exclusion criteria
* Inflammatory bowel disease * Colonic cancer * Bariatric surgery * Previous administration of antibiotic (within 15 days) * Pregnancy
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| rDNA comparison between cases and controls | 36 months | Comparison of 16sRDNA profile in cases and controls in order to identify bacteria associated with metabolic diseases that reside both in intestinal crypts and in blood. |
Countries
France
Outcome results
None listed