Isolation of Vitamin D Binding Protein Isoforms From Human Volunteers

Isolation of Genotype-specific Isoforms of Human Vitamin D-binding Protein (DBP/Gc)

Status

Active, not recruiting

Phases

Unknown

Study type

Observational

Source

ClinicalTrials.gov

Registry ID

NCT02258035

Enrollment

Registered

2014-10-07

Start date

2014-11-30

Completion date

2025-12-31

Last updated

2024-07-03

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Vitamin D Deficiency

Keywords

vitamin D binding protein (DBP)

Brief summary

The purpose of this study is to isolate genotype-specific DBP/Gc from homozygous human volunteers and use these purified proteins to cross-validate existing or novel assays for this serum protein and to evaluate the binding characteristics of these variants of DBP/Gc for different vitamin D metabolites.

Interventions

OTHERBlood draw

Sampling of 100-150 ml of blood

Study design

Observational model

CASE_ONLY

Time perspective

PROSPECTIVE

Eligibility

Sex/Gender

ALL

Age

18 Years to No maximum

Healthy volunteers

Yes

Inclusion criteria

* Generally healthy adults (≥ 18 years), non pregnant, with known DBP/Gc genotype

Exclusion criteria

* Inability to provide written informed consent * No children or pregnant women * Persons with known abnormal serum proteins

Design outcomes

Primary

Measure	Time frame	Description
Vitamin D binding protein binding characteristics (affinity constant)	single blood draw at time of consultation, an expected average 4 years after curative treatment for breast cancer	In vitro characterization

Secondary

Measure	Time frame	Description
Correlation coefficient (r) of different DBP assays	single blood draw at time of consultation, an expected average 4 years after curative treatment for breast cancer	In vitro characterization

Countries

Belgium

Outcome results

None listed

Source: ClinicalTrials.gov · Data processed: Feb 4, 2026