Non-obstructive Azoospermia With Testicular Biopsy for Spermatozoa Extraction
Conditions
Brief summary
Estradiol and Testosterone are two important hormones for the regulation of an effective spermatogenesis in human testis. The evaluation of levels of estradiol and testosterone in seminal plasma of men with non-obstructive azoospermia may be a predictive test before surgical testicular biopsy to determine the chance of a positive sperm extraction to use for ICSI.
Detailed description
The aim of this study is to compare the levels of esttradiol and testosterone in seminal plasma of men with non-obstructive azoospermia. Indeed, for these patients, none biological test allows to predict a positive sperm extraction before the surgical testis biopsy is performed. Since the involvement of estradiol and testosterone in the regulation of spermatogenesis, the measurement of these hormones in seminal plasma may be a predictive tool for these men before testis biopsy.
Interventions
OTHERESTRADIOL
Sponsors
University Hospital, Clermont-Ferrand
Study design
Time perspective
CROSS_SECTIONAL
Eligibility
Sex/Gender
MALE
Age
18 Years to 55 Years
Healthy volunteers
No
Inclusion criteria
* Azoospermia confirmed by two semen analysis (at minimum 74 days between the both analysis) * Volume of seminal plasma \>0.6ml * Testicular biopsy one month after the last semen analysis * Informed consent signed by the patient
Exclusion criteria
* obstructive azoospermia * cryptozoospermia
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Levels of estradiol and testosterone in seminal plasma of men with non-obstuctive azoospermia | at 1 month |
| Issue of testicular biopsy (sperm extraction positive or negative) | at day 1 |
Secondary
| Measure | Time frame |
|---|---|
| Degrees of alteration of testis histology | at day 1 |
| Serum levels of estradiol and testosterone | at day 1 |
Countries
France
Outcome results
None listed