Alzheimer Disease, Healthy Volunteers, Mild Cognitive Impairment, Parkinson Disease
Conditions
Keywords
MicroRNAs, exercise, skeletal muscle, metabolism
Brief summary
The purpose of this study is to determine specific changes in muscle secretory profile (myokines, miRNA) in association with neurodegenerative disease progression and metabolic dysfunction. Next the investigators would like to determine the shift in the muscle secretory activity induced by regular exercise intervention, which the investigators think could be translated into the beneficial changes in clinical phenotypes, determined by neuroimaging, cognitive function tests and metabolic phenotyping.
Detailed description
Standard Operating Procedures for patient recruitment, data collection, data management, data analysis routinely used in University Hospital Bratislava and in Institute of Experimental Endocrinology Slovak Academy of Sciences will be employed. Statistical analysis will be employed to address the primary and secondary objectives, as specified in the study protocol.
Interventions
BEHAVIORAL3 months exercise intervention program
3 months exercise intervention program which is given 3-times per week, 60 min session duration, 65-75% repetition maximum or heart rate maximum, combined strength & aerobic exercises
Sponsors
University Hospital Bratislava
Slovak Academy of Sciences
Study design
Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
PREVENTION
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
60 Years to 85 Years
Healthy volunteers
Yes
Inclusion criteria
1. Signed informed consent. 2. Diagnosed with Parkinson disease 3. Diagnosed with mild cognitive impairment (MCI) or Alzheimer disease (AD)
Exclusion criteria
1. Serious systemic cardiovascular, hepatic, renal disease, cancer. 2. Lack of compliance.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| muscle microRNAs and myokines | up to 24 months | We will determine muscle bioactive secretory products (myokines & miRNAs) that are regulated by acute bout of exercise in patients with neurodegenerative disease as well as in sedentary age, gender and BMI matched controls in association with their metabolic phenotype. |
| whole body energy metabolism | up to 24 months | changes in whole body energy metabolism will be determined with indirect calorimetry. & oral glucose tolerance test |
| cognitive function | up to 24 months | exercise related changes in cognitive & motoric functions will be determined with the aid of questionaires, and proton (1H)-magnetic resonance spectroscopy - brain imaging and spectroscopy |
| motoric function | up to 30 months | Proton (1H)-magnetic resonance spectroscopy - brain imaging and spectroscopy, motoric - balance testing |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Muscle functional tests | up to 30 months | Dynamometry will be used to determine maximal voluntary contraction force and the rate of force development, and Phosphorus (31P)-magnetic resonance spectroscopy to measure muscle metabolism in vivo. |
Countries
Slovakia
Outcome results
None listed