Healthy
Conditions
Keywords
Brazilian fruit peel, Functional food, Cardiometabolic risk, Metabolic syndrome, Inflammation, Appetite, Gut hormone
Brief summary
The purpose of the study was to investigate how Brazilian fruits peel affect acute/postprandial glucose and insulin responses, inflammatory markers, appetite control peptides, antioxidative capacity, as well as subjective appetite ratings (VAS-visual analogue scales) in healthy volunteers. We hypothesize that certain Brazilian fruits peel added to a standardized meal will improve postprandial glucose tolerance and other metabolic biomarkers in healthy volunteers, compared with a similar meal without the corresponding plant materials.
Interventions
DIETARY_SUPPLEMENTControl drink
As a control, subjects are asked to consume 300 ml control drinks containing equal amount of total fiber and pectine as Brazilian fruit drink. Following 10 min. beverage consumption, subjects will be given standardized breakfast corresponding to 50 g carbohydrate.
DIETARY_SUPPLEMENTBrazilian fruit peel flour
In this study, subjects are asked to consume 300 ml beverage drinks contain certain amounts of Brazilian fruit peel flour. Following 10 min. beverage consumption, subjects will be given standardized breakfast corresponding to 50 g carbohydrate.
DIETARY_SUPPLEMENTNegative control drink
As a negative control, subjects are asked to consume 300 ml control drinks without containing amount of total fiber and pectine as Brazilian fruit drink. Following 10 min. beverage consumption, subjects will be given standardized breakfast corresponding to 50 g carbohydrate.
Sponsors
University of Campinas, Brazil
Lund University
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
SINGLE (Subject)
Eligibility
Sex/Gender
ALL
Age
18 Years to 40 Years
Healthy volunteers
Yes
Inclusion criteria
* Healthy males and females * Signed informed consent * BMI 20 - 28 kg/m2 with weight change \<3 kg latest 2 months * Must be able to accept plant-based foods/drinks
Exclusion criteria
* Below 18 years * Uncomfortable speaking English and/or difficulties in understanding spoken English * Smoking or using snuff * Vegetarian or vegan * Stressed by venous blood sampling or previous experience of being difficult to cannulate * Receiving any drug treatment that may influence the study outcomes * Pregnancy or breastfeeding
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Changing on blood glucose concentration after treatment with brazilian fruits peel | Prior to the initial intervention at 0 min and after intervention at 15, 30, 45, 60, 90, 120, 180 min | The capillary blood samples will be taken for blood glucose analysis |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Changing on subjective appetite ratings after treatment with Brazilian fruits peel | Prior to the initial intervention at 0 min and after intervention at 15, 30, 45, 60, 90, 120, 180 min. | Appetite will be assessed using standard subjective 100 mm VAS (visual analogue scale) at time intervals throughout each visit |
| Identify plasma metabolite profile (untargeted approach) after treatment with Brazilian fruits peel | Prior to the initial intervention at 0 min and after intervention at 60, 90, 120, 180 min. | Metabolite profile will be analyzed using Liquid Chromatography-Mass Spectrometry based methods |
| Changing on insulin after treatment with Brazilian fruits peel | Prior to the initial intervention at 0 min and after intervention at 15, 30, 45, 60, 90, 120, 180 min. | The venous serum blood samples will be taken for insulin analysis |
| Changing on inflammatory markers (i.e. interleukin-6, interleukin-8, tumor necrosis factor-alpha, necrosis factor kappa-beta, adiponectin and C-reactive protein) after treatment with Brazilian fruits peel | Prior to the initial intervention at 0 min and after intervention at 15, 30, 45, 60, 90, 120, 180 min. | The venous serum blood samples will be taken for inflammatory markers analysis |
| Changing on appetite markers (i.e. ghrelin, cholecystokinin, glucagon-like peptide-1, gastric inhibitory polypeptide, peptide YY, amylin, pancreatic polypeptide) after treatment with Brazilian fruits peel | Prior to the initial intervention at 0 min and after intervention at 15, 30, 45, 60, 90, 120, 180 min. | The venous serum blood samples will be taken for appetite markers analysis |
Countries
Sweden
Outcome results
None listed