Asthma
Conditions
Keywords
Asthma, Allergen challenge
Brief summary
This protocol describes a single site mechanistic study to investigate microRNAs (miRNAs) that are differentially expressed in the airway epithelium of patients with asthma at baseline and in response to allergen challenge. We hypothesize that allergen exposure enhances airway smooth muscle contractility and epithelial cell mRNA/miRNA production as a consequence of locally increased T-cell derived cytokine production. The study will involve three visits over the course of approximately 14 days. At Visit 1, participants will be characterized in detail with lung function testing, methacholine challenge testing, and allergen skin prick testing. At Visit 2, participants will undergo bronchoscopy with segmental allergen administration of either cat or dust mite standardized allergen extract. At Visit 3 (either 24 hours later or 7 days later), bronchoscopy will be performed to collect airway samples including bronchoalveolar lavage (BAL), epithelial brushings and endobronchial biopsies. Sample analysis will include measurement of miRNA and mRNA expression in epithelial brushings (RNAseq and qPCR); analysis of cell surface markers on BAL cells and blood cells; and collection of endobronchial biopsies for immunostaining of immune cells localization, immunoblotting of smooth cell protein phosphorylation, analysis of mucin content and smooth muscle cell subculture. A total of 38 subjects (26 asthmatics with stable or well-controlled asthma, 6 allergic non-asthmatics and 6 non-allergic non-asthmatics) will complete the study.
Interventions
BIOLOGICALSegmental airway allergen challenge
PROCEDUREBronchoscopy
DIAGNOSTIC_TESTMethacholine challenge test
DIAGNOSTIC_TESTSpirometry
DIAGNOSTIC_TESTAllergen skin prick test
Sponsors
National Institute of Allergy and Infectious Diseases (NIAID)
Prescott Woodruff
Study design
Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
BASIC_SCIENCE
Masking
NONE
Intervention model description
Single group before and after administration of allergen
Eligibility
Sex/Gender
ALL
Age
18 Years to 50 Years
Healthy volunteers
Yes
Inclusion criteria
Allergic/Non-Asthmatic subjects and Allergic/Asthmatic subjects Inclusion Criteria: * Positive skin test to dust mite or cat allergen Non-Allergic/Non-Asthmatic subjects Inclusion Criteria: * Negative skin test to panel of 12 allergens, including dust mite and cat allergen All groups
Exclusion criteria
* History of intubation for asthma exacerbation * Use of Xolair (omalizumab) within the last 6 months * Immunotherapy with cat or dust mite extract now or in the past 5 years * ≥ 10 pack-years smoking or any smoking in the past year * Other lung diseases, such as sarcoidosis, bronchiectasis or active lung infection * History of dermatographia * History of anaphylaxis to cat allergen * Participation in another research study involving a drug or biologic during the past 30 days * Presence of past or current medical problems/other factors that may pose additional risks from participation or influence study results, as determined per study investigator
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Difference in Expression of Epithelial miRNAs (in Read Counts) 24 Hours After Allergen-challenge as Compared to 24 Hours After Diluent Challenge in Participants With Allergic Asthma | 24 hours | For each participant with Allergic Asthma, a baseline airway epithelial brushing was performed, allergen was instilled into one segment of the lung, and diluent (control) was instilled into another segment. Both segments were brushed for epithelial cells 24 hours later. After processing brushes to RNA and generating RNA sequencing data, the RNA transcript read counts for each gene underwent variance stabilized log normalization. Then we built a regression model which estimated the difference in miRNA read count in the allergen challenged as compared to the baseline sample and the difference in miRNA read count in the diluent challenged segment as compared to baseline for each participant. The change from baseline read count for each miRNA in the allergen samples was compared to the change in the diluent samples and statistical significance was assessed using the unadjusted p-value(\<0.05). The direction of change does not have clinical significance; this was a mechanistic study. |
| Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | 7 days | For each participant with Allergic Asthma, a baseline airway epithelial brushing was performed, allergen was instilled into one segment of the lung, and diluent (control) was instilled into another segment. Both segments were brushed for epithelial cells 7 days later. After processing the brushes to RNA and generating RNA sequencing data, the RNA transcript read counts for each gene underwent variance stabilized log normalization. Then we built a regression model which estimated the difference in miRNA read count in the allergen challenged as compared to the baseline sample and the difference in miRNA read count in the diluent challenged segment as compared to baseline for each participant. The change from baseline read count for each miRNA in the allergen samples was compared to the change in the diluent samples and statistical significance was assessed using the unadjusted p-value(\<0.05). The direction of change does not have clinical significance; this was a mechanistic study. |
Countries
United States
Participant flow
Participants by arm
| Arm | Count |
|---|---|
| Allergic Non-asthmatics(ANA) ) Volunteers with allergy (as established by skin prick testing) but without asthma | 2 |
| Non-allergic Non-asthmatics(NANA) Volunteers with neither asthma nor allergy (as established by skin prick testing) | 3 |
| Allergic Asthmatics(AA) Volunteers with both asthma and allergy (as established by skin prick testing) | 17 |
| Total | 22 |
Withdrawals & dropouts
| Period | Reason | FG000 | FG001 | FG002 |
|---|---|---|---|---|
| Overall Study | Lost to Follow-up | 1 | 0 | 0 |
| Overall Study | Physician Decision | 1 | 0 | 0 |
| Overall Study | Withdrawal by Subject | 3 | 0 | 1 |
Baseline characteristics
| Characteristic | Allergic Non-asthmatics(ANA) | Total | Allergic Asthmatics(AA) | Non-allergic Non-asthmatics(NANA) |
|---|---|---|---|---|
| Age, Categorical <=18 years | 0 Participants | 1 Participants | 1 Participants | 0 Participants |
| Age, Categorical >=65 years | 0 Participants | 0 Participants | 0 Participants | 0 Participants |
| Age, Categorical Between 18 and 65 years | 2 Participants | 21 Participants | 16 Participants | 3 Participants |
| Age, Continuous | 29.5 years STANDARD_DEVIATION 2.12 | 29.5 years STANDARD_DEVIATION 9.268 | 29.7 years STANDARD_DEVIATION 7.15 | 29 years STANDARD_DEVIATION 5 |
| Ethnicity (NIH/OMB) Hispanic or Latino | 1 Participants | 5 Participants | 4 Participants | 0 Participants |
| Ethnicity (NIH/OMB) Not Hispanic or Latino | 1 Participants | 17 Participants | 13 Participants | 3 Participants |
| Ethnicity (NIH/OMB) Unknown or Not Reported | 0 Participants | 0 Participants | 0 Participants | 0 Participants |
| FEV1 | 4.52 liters STANDARD_DEVIATION 1.71 | 3.6 liters STANDARD_DEVIATION 0.864 | 3.38 liters STANDARD_DEVIATION 0.789 | 4.22 liters STANDARD_DEVIATION 0.397 |
| Race (NIH/OMB) American Indian or Alaska Native | 0 Participants | 0 Participants | 0 Participants | 0 Participants |
| Race (NIH/OMB) Asian | 0 Participants | 4 Participants | 3 Participants | 1 Participants |
| Race (NIH/OMB) Black or African American | 0 Participants | 3 Participants | 2 Participants | 1 Participants |
| Race (NIH/OMB) More than one race | 0 Participants | 0 Participants | 0 Participants | 0 Participants |
| Race (NIH/OMB) Native Hawaiian or Other Pacific Islander | 0 Participants | 1 Participants | 1 Participants | 0 Participants |
| Race (NIH/OMB) Unknown or Not Reported | 0 Participants | 3 Participants | 3 Participants | 0 Participants |
| Race (NIH/OMB) White | 2 Participants | 11 Participants | 8 Participants | 1 Participants |
| Region of Enrollment United States | 2 participants | 22 participants | 17 participants | 3 participants |
| Sex: Female, Male Female | 1 Participants | 13 Participants | 11 Participants | 1 Participants |
| Sex: Female, Male Male | 1 Participants | 9 Participants | 6 Participants | 2 Participants |
Adverse events
| Event type | EG000 affected / at risk | EG001 affected / at risk | EG002 affected / at risk |
|---|---|---|---|
| deaths Total, all-cause mortality | 0 / 22 | 0 / 2 | 0 / 4 |
| other Total, other adverse events | 2 / 22 | 0 / 2 | 0 / 4 |
| serious Total, serious adverse events | 1 / 22 | 0 / 2 | 0 / 4 |
Outcome results
Primary
Difference in Expression of Epithelial miRNAs (in Read Counts) 24 Hours After Allergen-challenge as Compared to 24 Hours After Diluent Challenge in Participants With Allergic Asthma
For each participant with Allergic Asthma, a baseline airway epithelial brushing was performed, allergen was instilled into one segment of the lung, and diluent (control) was instilled into another segment. Both segments were brushed for epithelial cells 24 hours later. After processing brushes to RNA and generating RNA sequencing data, the RNA transcript read counts for each gene underwent variance stabilized log normalization. Then we built a regression model which estimated the difference in miRNA read count in the allergen challenged as compared to the baseline sample and the difference in miRNA read count in the diluent challenged segment as compared to baseline for each participant. The change from baseline read count for each miRNA in the allergen samples was compared to the change in the diluent samples and statistical significance was assessed using the unadjusted p-value(\<0.05). The direction of change does not have clinical significance; this was a mechanistic study.
Time frame: 24 hours
Population: Our pre-specified primary outcome was limited to Allergic Asthmatics only. The rationale for this is that participants without allergy or asthma do not respond to Allergen Challenge and therefore the changes in miRNA are uninformative.
| Arm | Measure | Group | Value (MEAN) | Dispersion |
|---|---|---|---|---|
| Allergen Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 24 Hours After Allergen-challenge as Compared to 24 Hours After Diluent Challenge in Participants With Allergic Asthma | miR4521 | 7.33 Normalized log2 copy number | Standard Deviation 0.79 |
| Allergen Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 24 Hours After Allergen-challenge as Compared to 24 Hours After Diluent Challenge in Participants With Allergic Asthma | miR107 | 9.63 Normalized log2 copy number | Standard Deviation 0.61 |
| Allergen Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 24 Hours After Allergen-challenge as Compared to 24 Hours After Diluent Challenge in Participants With Allergic Asthma | miR891a | 7.02 Normalized log2 copy number | Standard Deviation 0.64 |
| Allergen Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 24 Hours After Allergen-challenge as Compared to 24 Hours After Diluent Challenge in Participants With Allergic Asthma | miR363 | 9.55 Normalized log2 copy number | Standard Deviation 1.16 |
| Allergen Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 24 Hours After Allergen-challenge as Compared to 24 Hours After Diluent Challenge in Participants With Allergic Asthma | miR1248 | 7.02 Normalized log2 copy number | Standard Deviation 0.31 |
| Allergen Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 24 Hours After Allergen-challenge as Compared to 24 Hours After Diluent Challenge in Participants With Allergic Asthma | miR944 | 7.12 Normalized log2 copy number | Standard Deviation 0.19 |
| Allergen Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 24 Hours After Allergen-challenge as Compared to 24 Hours After Diluent Challenge in Participants With Allergic Asthma | miR6806 | 6.63 Normalized log2 copy number | Standard Deviation 0.22 |
| Allergen Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 24 Hours After Allergen-challenge as Compared to 24 Hours After Diluent Challenge in Participants With Allergic Asthma | miR103a | 12.05 Normalized log2 copy number | Standard Deviation 0.38 |
| Allergen Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 24 Hours After Allergen-challenge as Compared to 24 Hours After Diluent Challenge in Participants With Allergic Asthma | miR454-5p | 6.77 Normalized log2 copy number | Standard Deviation 0.16 |
| Allergen Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 24 Hours After Allergen-challenge as Compared to 24 Hours After Diluent Challenge in Participants With Allergic Asthma | miR320e | 6.57 Normalized log2 copy number | Standard Deviation 0.16 |
| Allergen Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 24 Hours After Allergen-challenge as Compared to 24 Hours After Diluent Challenge in Participants With Allergic Asthma | miR181a | 8.29 Normalized log2 copy number | Standard Deviation 0.33 |
| Allergen Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 24 Hours After Allergen-challenge as Compared to 24 Hours After Diluent Challenge in Participants With Allergic Asthma | miR130b | 6.72 Normalized log2 copy number | Standard Deviation 0.17 |
| Allergen Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 24 Hours After Allergen-challenge as Compared to 24 Hours After Diluent Challenge in Participants With Allergic Asthma | miR365a | 6.4 Normalized log2 copy number | Standard Deviation 0.14 |
| Allergen Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 24 Hours After Allergen-challenge as Compared to 24 Hours After Diluent Challenge in Participants With Allergic Asthma | miR135b | 6.65 Normalized log2 copy number | Standard Deviation 0.22 |
| Allergen Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 24 Hours After Allergen-challenge as Compared to 24 Hours After Diluent Challenge in Participants With Allergic Asthma | miR1273h | 6.53 Normalized log2 copy number | Standard Deviation 0.24 |
| Allergen Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 24 Hours After Allergen-challenge as Compared to 24 Hours After Diluent Challenge in Participants With Allergic Asthma | miR3177 | 6.79 Normalized log2 copy number | Standard Deviation 0.26 |
| Diluent Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 24 Hours After Allergen-challenge as Compared to 24 Hours After Diluent Challenge in Participants With Allergic Asthma | miR3177 | 6.88 Normalized log2 copy number | Standard Deviation 0.27 |
| Diluent Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 24 Hours After Allergen-challenge as Compared to 24 Hours After Diluent Challenge in Participants With Allergic Asthma | miR4521 | 6.74 Normalized log2 copy number | Standard Deviation 0.43 |
| Diluent Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 24 Hours After Allergen-challenge as Compared to 24 Hours After Diluent Challenge in Participants With Allergic Asthma | miR454-5p | 6.57 Normalized log2 copy number | Standard Deviation 0.28 |
| Diluent Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 24 Hours After Allergen-challenge as Compared to 24 Hours After Diluent Challenge in Participants With Allergic Asthma | miR107 | 9.01 Normalized log2 copy number | Standard Deviation 0.98 |
| Diluent Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 24 Hours After Allergen-challenge as Compared to 24 Hours After Diluent Challenge in Participants With Allergic Asthma | miR365a | 6.55 Normalized log2 copy number | Standard Deviation 0.21 |
| Diluent Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 24 Hours After Allergen-challenge as Compared to 24 Hours After Diluent Challenge in Participants With Allergic Asthma | miR891a | 6.56 Normalized log2 copy number | Standard Deviation 0.46 |
| Diluent Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 24 Hours After Allergen-challenge as Compared to 24 Hours After Diluent Challenge in Participants With Allergic Asthma | miR320e | 6.69 Normalized log2 copy number | Standard Deviation 0.24 |
| Diluent Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 24 Hours After Allergen-challenge as Compared to 24 Hours After Diluent Challenge in Participants With Allergic Asthma | miR363 | 8.77 Normalized log2 copy number | Standard Deviation 1.07 |
| Diluent Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 24 Hours After Allergen-challenge as Compared to 24 Hours After Diluent Challenge in Participants With Allergic Asthma | miR1273h | 6.62 Normalized log2 copy number | Standard Deviation 0.33 |
| Diluent Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 24 Hours After Allergen-challenge as Compared to 24 Hours After Diluent Challenge in Participants With Allergic Asthma | miR1248 | 7.21 Normalized log2 copy number | Standard Deviation 0.48 |
| Diluent Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 24 Hours After Allergen-challenge as Compared to 24 Hours After Diluent Challenge in Participants With Allergic Asthma | miR181a | 8.83 Normalized log2 copy number | Standard Deviation 0.86 |
| Diluent Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 24 Hours After Allergen-challenge as Compared to 24 Hours After Diluent Challenge in Participants With Allergic Asthma | miR944 | 7.27 Normalized log2 copy number | Standard Deviation 0.36 |
| Diluent Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 24 Hours After Allergen-challenge as Compared to 24 Hours After Diluent Challenge in Participants With Allergic Asthma | miR135b | 6.43 Normalized log2 copy number | Standard Deviation 0.19 |
| Diluent Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 24 Hours After Allergen-challenge as Compared to 24 Hours After Diluent Challenge in Participants With Allergic Asthma | miR6806 | 6.35 Normalized log2 copy number | Standard Deviation 0.13 |
| Diluent Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 24 Hours After Allergen-challenge as Compared to 24 Hours After Diluent Challenge in Participants With Allergic Asthma | miR130b | 6.5 Normalized log2 copy number | Standard Deviation 0.33 |
| Diluent Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 24 Hours After Allergen-challenge as Compared to 24 Hours After Diluent Challenge in Participants With Allergic Asthma | miR103a | 11.7 Normalized log2 copy number | Standard Deviation 0.48 |
Comparison: miR4521p-value: 0.01t-test, 2 sided
Comparison: miR107p-value: 0.01t-test, 2 sided
Comparison: miR-891ap-value: 0.018t-test, 2 sided
Comparison: miR363p-value: 0.021t-test, 2 sided
Comparison: miR1248p-value: 0.028t-test, 2 sided
Comparison: miR944p-value: 0.033t-test, 2 sided
Comparison: miR6806p-value: 0.034t-test, 2 sided
Comparison: miR103ap-value: 0.036t-test, 2 sided
Comparison: miR454p-value: 0.037t-test, 2 sided
Comparison: miR320ep-value: 0.041t-test, 2 sided
Comparison: miR181ap-value: 0.043t-test, 2 sided
Comparison: miR130bp-value: 0.044t-test, 2 sided
Comparison: miR365ap-value: 0.044t-test, 2 sided
Comparison: miR135bp-value: 0.047t-test, 2 sided
Comparison: miR1273hp-value: 0.048t-test, 2 sided
Comparison: miR3177p-value: 0.048t-test, 2 sided
Primary
Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge
For each participant with Allergic Asthma, a baseline airway epithelial brushing was performed, allergen was instilled into one segment of the lung, and diluent (control) was instilled into another segment. Both segments were brushed for epithelial cells 7 days later. After processing the brushes to RNA and generating RNA sequencing data, the RNA transcript read counts for each gene underwent variance stabilized log normalization. Then we built a regression model which estimated the difference in miRNA read count in the allergen challenged as compared to the baseline sample and the difference in miRNA read count in the diluent challenged segment as compared to baseline for each participant. The change from baseline read count for each miRNA in the allergen samples was compared to the change in the diluent samples and statistical significance was assessed using the unadjusted p-value(\<0.05). The direction of change does not have clinical significance; this was a mechanistic study.
Time frame: 7 days
Population: Our pre-specified primary outcome was limited to Allergic Asthmatics only. The rationale for this is that participants without allergy or asthma do not respond to Allergen Challenge and therefore the changes in miRNA are uninformative.
| Arm | Measure | Group | Value (MEAN) | Dispersion |
|---|---|---|---|---|
| Allergen Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR193a | 6.66 Normalized log2 copy number | Standard Deviation 0.99 |
| Allergen Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR320d | 6.48 Normalized log2 copy number | Standard Deviation 0.33 |
| Allergen Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR3928 | 5.95 Normalized log2 copy number | Standard Deviation 0.3 |
| Allergen Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR744 | 5.6 Normalized log2 copy number | Standard Deviation 0.58 |
| Allergen Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR206 | 5.67 Normalized log2 copy number | Standard Deviation 1.06 |
| Allergen Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR30e | 11.3 Normalized log2 copy number | Standard Deviation 0.29 |
| Allergen Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR4500 | 6.21 Normalized log2 copy number | Standard Deviation 0.41 |
| Allergen Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR125a | 13.66 Normalized log2 copy number | Standard Deviation 1.47 |
| Allergen Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR99b | 13.78 Normalized log2 copy number | Standard Deviation 0.31 |
| Allergen Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR1 | 5.86 Normalized log2 copy number | Standard Deviation 0.76 |
| Allergen Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR146b | 14.13 Normalized log2 copy number | Standard Deviation 0.38 |
| Allergen Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR1255a | 5.21 Normalized log2 copy number | Standard Deviation 0.54 |
| Allergen Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR223 | 9.9 Normalized log2 copy number | Standard Deviation 0.6 |
| Allergen Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR642a | 7.8 Normalized log2 copy number | Standard Deviation 0.56 |
| Allergen Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR548ae | 5.86 Normalized log2 copy number | Standard Deviation 0.24 |
| Allergen Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR146b-3p | 7.9 Normalized log2 copy number | Standard Deviation 0.35 |
| Allergen Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR877 | 5.13 Normalized log2 copy number | Standard Deviation 0.42 |
| Allergen Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR181a | 8.25 Normalized log2 copy number | Standard Deviation 0.32 |
| Allergen Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR4467 | 5.17 Normalized log2 copy number | Standard Deviation 0.47 |
| Allergen Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR1249 | 5.05 Normalized log2 copy number | Standard Deviation 0.35 |
| Allergen Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR766 | 5.59 Normalized log2 copy number | Standard Deviation 0.63 |
| Allergen Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR29c | 7.93 Normalized log2 copy number | Standard Deviation 0.56 |
| Allergen Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR370 | 5.77 Normalized log2 copy number | Standard Deviation 0.76 |
| Allergen Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR6510 | 7.74 Normalized log2 copy number | Standard Deviation 0.57 |
| Allergen Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR3605 | 5.92 Normalized log2 copy number | Standard Deviation 0.61 |
| Allergen Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR3912 | 5.03 Normalized log2 copy number | Standard Deviation 0.53 |
| Allergen Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR15b | 6.76 Normalized log2 copy number | Standard Deviation 0.33 |
| Allergen Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR127 | 7.06 Normalized log2 copy number | Standard Deviation 0.8 |
| Allergen Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR146a | 14.14 Normalized log2 copy number | Standard Deviation 1.19 |
| Allergen Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR449b | 9.99 Normalized log2 copy number | Standard Deviation 0.54 |
| Allergen Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR21-5p | 18.02 Normalized log2 copy number | Standard Deviation 0.33 |
| Allergen Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR6842 | 6.83 Normalized log2 copy number | Standard Deviation 0.37 |
| Allergen Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR142-5p | 7.68 Normalized log2 copy number | Standard Deviation 0.38 |
| Allergen Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR21 | 7.97 Normalized log2 copy number | Standard Deviation 0.49 |
| Allergen Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR493 | 5.03 Normalized log2 copy number | Standard Deviation 0.31 |
| Allergen Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR149 | 9.98 Normalized log2 copy number | Standard Deviation 0.42 |
| Allergen Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR449a | 12.46 Normalized log2 copy number | Standard Deviation 0.56 |
| Allergen Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR6511a | 5.58 Normalized log2 copy number | Standard Deviation 0.66 |
| Allergen Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR6843 | 5.99 Normalized log2 copy number | Standard Deviation 0.11 |
| Allergen Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR671 | 8.53 Normalized log2 copy number | Standard Deviation 0.87 |
| Allergen Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR6503 | 4.93 Normalized log2 copy number | Standard Deviation 0.19 |
| Allergen Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR450b | 6.05 Normalized log2 copy number | Standard Deviation 0.4 |
| Allergen Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR485 | 5.08 Normalized log2 copy number | Standard Deviation 0.62 |
| Allergen Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR6806 | 5.22 Normalized log2 copy number | Standard Deviation 0.38 |
| Allergen Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR3182 | 5.92 Normalized log2 copy number | Standard Deviation 0.2 |
| Allergen Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR142-3p | 7.58 Normalized log2 copy number | Standard Deviation 0.21 |
| Diluent Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR6510 | 9.2 Normalized log2 copy number | Standard Deviation 0.81 |
| Diluent Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR193a | 7.46 Normalized log2 copy number | Standard Deviation 0.72 |
| Diluent Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR320d | 7.09 Normalized log2 copy number | Standard Deviation 0.72 |
| Diluent Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR3605 | 6.9 Normalized log2 copy number | Standard Deviation 0.8 |
| Diluent Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR3928 | 5.24 Normalized log2 copy number | Standard Deviation 0.49 |
| Diluent Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR485 | 5.68 Normalized log2 copy number | Standard Deviation 0.72 |
| Diluent Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR744 | 5.1 Normalized log2 copy number | Standard Deviation 0.47 |
| Diluent Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR3912 | 5.83 Normalized log2 copy number | Standard Deviation 0.61 |
| Diluent Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR206 | 5.18 Normalized log2 copy number | Standard Deviation 0.61 |
| Diluent Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR149 | 10.95 Normalized log2 copy number | Standard Deviation 0.4 |
| Diluent Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR30e | 10.36 Normalized log2 copy number | Standard Deviation 0.7 |
| Diluent Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR15b | 6.09 Normalized log2 copy number | Standard Deviation 0.39 |
| Diluent Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR4500 | 5.61 Normalized log2 copy number | Standard Deviation 0.22 |
| Diluent Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR6503 | 5.56 Normalized log2 copy number | Standard Deviation 0.59 |
| Diluent Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR125a | 14.55 Normalized log2 copy number | Standard Deviation 1.18 |
| Diluent Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR127 | 8.54 Normalized log2 copy number | Standard Deviation 1.73 |
| Diluent Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR99b | 14.33 Normalized log2 copy number | Standard Deviation 0.4 |
| Diluent Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR449a | 11.36 Normalized log2 copy number | Standard Deviation 0.95 |
| Diluent Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR1 | 5.17 Normalized log2 copy number | Standard Deviation 0.49 |
| Diluent Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR146a | 12.73 Normalized log2 copy number | Standard Deviation 0.8 |
| Diluent Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR146b | 14.72 Normalized log2 copy number | Standard Deviation 0.76 |
| Diluent Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR3182 | 5.68 Normalized log2 copy number | Standard Deviation 0.97 |
| Diluent Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR1255a | 5.79 Normalized log2 copy number | Standard Deviation 0.67 |
| Diluent Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR449b | 8.84 Normalized log2 copy number | Standard Deviation 0.88 |
| Diluent Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR223 | 8.9 Normalized log2 copy number | Standard Deviation 1.08 |
| Diluent Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR6511a | 6.46 Normalized log2 copy number | Standard Deviation 0.56 |
| Diluent Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR642a | 8.32 Normalized log2 copy number | Standard Deviation 0.22 |
| Diluent Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR21-5p | 17 Normalized log2 copy number | Standard Deviation 0.82 |
| Diluent Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR548ae | 5.23 Normalized log2 copy number | Standard Deviation 0.4 |
| Diluent Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR450b | 5.35 Normalized log2 copy number | Standard Deviation 0.52 |
| Diluent Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR146b-3p | 8.58 Normalized log2 copy number | Standard Deviation 0.31 |
| Diluent Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR6842 | 7.67 Normalized log2 copy number | Standard Deviation 0.44 |
| Diluent Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR877 | 5.66 Normalized log2 copy number | Standard Deviation 0.39 |
| Diluent Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR6843 | 6.8 Normalized log2 copy number | Standard Deviation 0.67 |
| Diluent Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR181a | 8.74 Normalized log2 copy number | Standard Deviation 0.58 |
| Diluent Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR142-5p | 6.93 Normalized log2 copy number | Standard Deviation 0.66 |
| Diluent Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR4467 | 5.56 Normalized log2 copy number | Standard Deviation 0.92 |
| Diluent Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR6806 | 5.85 Normalized log2 copy number | Standard Deviation 0.43 |
| Diluent Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR1249 | 5.62 Normalized log2 copy number | Standard Deviation 0.44 |
| Diluent Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR21 | 6.83 Normalized log2 copy number | Standard Deviation 0.99 |
| Diluent Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR766 | 4.98 Normalized log2 copy number | Standard Deviation 0.3 |
| Diluent Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR671 | 9.3 Normalized log2 copy number | Standard Deviation 0.73 |
| Diluent Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR29c | 7.09 Normalized log2 copy number | Standard Deviation 0.78 |
| Diluent Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR493 | 5.66 Normalized log2 copy number | Standard Deviation 0.67 |
| Diluent Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR370 | 6.51 Normalized log2 copy number | Standard Deviation 1 |
| Diluent Challenge Subjects | Difference in Expression of Epithelial miRNAs (in Read Counts) 7 Days After Allergen-challenge as Compared to 7 Days After Diluent Challenge | miR142-3p | 6.72 Normalized log2 copy number | Standard Deviation 0.91 |
Comparison: miR6510p-value: 0t-test, 2 sided
Comparison: miR3605p-value: 0t-test, 2 sided
Comparison: miR3912p-value: 0t-test, 2 sided
Comparison: miR15bp-value: 0t-test, 2 sided
Comparison: miR127p-value: 0t-test, 2 sided
Comparison: miR146ap-value: 0t-test, 2 sided
Comparison: miR449bp-value: 0t-test, 2 sided
Comparison: miR21-5pp-value: 0.01t-test, 2 sided
Comparison: miR6842p-value: 0.01t-test, 2 sided
Comparison: miR142-5pp-value: 0.01t-test, 2 sided
Comparison: miR21p-value: 0.01t-test, 2 sided
Comparison: miR493p-value: 0.01t-test, 2 sided
Comparison: miR193ap-value: 0.01t-test, 2 sided
Comparison: miR149p-value: 0.01t-test, 2 sided
Comparison: miR449ap-value: 0.01t-test, 2 sided
Comparison: miR6511ap-value: 0.01t-test, 2 sided
Comparison: miR6843p-value: 0.01t-test, 2 sided
Comparison: miR671p-value: 0.01t-test, 2 sided
Comparison: miR6503p-value: 0.02t-test, 2 sided
Comparison: miR450bp-value: 0.02t-test, 2 sided
Comparison: miR485p-value: 0.02t-test, 2 sided
Comparison: miR6806p-value: 0.02t-test, 2 sided
Comparison: miR3182p-value: 0.02t-test, 2 sided
Comparison: miR142-3pp-value: 0.02t-test, 2 sided
Comparison: miR320dp-value: 0.02t-test, 2 sided
Comparison: miR3928p-value: 0.02t-test, 2 sided
Comparison: miR744p-value: 0.02t-test, 2 sided
Comparison: miR206p-value: 0.02t-test, 2 sided
Comparison: miR30ep-value: 0.03t-test, 2 sided
Comparison: miR4500p-value: 0.03t-test, 2 sided
Comparison: miR125ap-value: 0.03t-test, 2 sided
Comparison: miR99bp-value: 0.03t-test, 2 sided
Comparison: miR1p-value: 0.03t-test, 2 sided
Comparison: miR146bp-value: 0.03t-test, 2 sided
Comparison: miR1255ap-value: 0.03t-test, 2 sided
Comparison: miR223p-value: 0.03t-test, 2 sided
Comparison: miR642ap-value: 0.03t-test, 2 sided
Comparison: miR548aep-value: 0.4t-test, 2 sided
Comparison: miR146bp-value: 0.04t-test, 2 sided
Comparison: miR877p-value: 0.04t-test, 2 sided
Comparison: miR181ap-value: 0.04t-test, 2 sided
Comparison: miR4467p-value: 0.04t-test, 2 sided
Comparison: miR1249p-value: 0.04t-test, 2 sided
Comparison: miR766p-value: 0.04t-test, 2 sided
Comparison: miR29cp-value: 0.048t-test, 2 sided
Comparison: miR370p-value: -1.75t-test, 2 sided