Asthma, Healthy
Conditions
Keywords
healthy volunteers, asthma, sputum eosinophils
Brief summary
Can a clinical test be developed that could help manage asthma symptoms?
Detailed description
Project goals: Develop a point-of-service assay to assess eosinophilic airway inflammation Proposal Specific Aims: 1. To establish the infrastructure and test the feasibility of sputum collection in a clinical setting as a minimally invasive patient sampling process for biomarker testing 2. To transform the laboratory-based eosinophil peroxidase (EPX) ELISA detection assay into a clinic-friendly assessment that does not require sophisticated laboratory equipment
Interventions
None listed
Sponsors
Mayo Clinic
Study design
Observational model
CASE_CONTROL
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
Yes
Inclusion criteria
Asthma subjects: Inclusion * Provider diagnosed asthma * FeNO\>50 ppb * FEV1 \> 1.0 Liter Exclusion * COPD by history * Bronchiectasis by history * Pregnant participants Normal subjects: Inclusion \- Age matched to asthma subjects Exclusion * Any eosinophil disorder by history * Asthma by history * Rhinitis or sinusitis by history
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Sputum Induction | 10 minutes | Primary Outcome measure via EPX levels (at point of care) in the sputum of asthma patients and control subjects as a means of demonstrating the ability to use this biomarker approach in a clinical setting. |
Countries
United States
Outcome results
None listed