Disorder of Glucose Regulation
Conditions
Keywords
SGLT1 (sodium dependent glucose transporter), Glut-transporters, taste receptors genes, sweet taste receptor T1R2T1R3, bitter taste receptors of the hTAS2gene family
Brief summary
We investigate the impact of taste receptors (sweet, bitter and umami) on human metabolism. There ist growing evidence, that components of the taste signalling system, including the taste receptor proteins , have important functions in the regulation of metabolic parameters in non-gustatory tissues.
Detailed description
We apply different sweet, bitter and umami taste compounds in a single dose in the human intervention. We use substances as for instance glucose, sucrose,saccharin and lactisol in the sweet part, and substances from cabbage, beer or red endive in the bitter part. For umami we take glutamate. Blood samples are collected at baseline and 6 times thereafter up to 3hours, to analyse the incretins GLP-1(glucagon-like peptide 1) and GIP(gastrointestinal polypeptide) and other peptide hormones as insulin and PYY(peptide tyrosin trosin) in human beings. Gastrointestinal mucosa is taken by gastroscopy from the lower duodenum from study subjects for analysis for the presence or absence of functional taste receptors in gastrointestinal tissues.
Interventions
DIETARY_SUPPLEMENTsweet block
oral application of the above mentioned substances in 300 ml tap water in 5 min. , collection of blood at 0,15,30, 60 ,120,180 minutes for measurement of incretins GIP(gastrointestinal polypeptide) and GLP-1 (glucagon like peptide 1), Protein hormones PYY(Peptide YY),CCK (cholecystokinin), Leptin and Insulin
DIETARY_SUPPLEMENTbitter block
oral application of the above mentioned substances in 300 ml tap water, collection of blood samples at -15,0,15,30,60,120,180 min for Insulin, GIP,GLP-1, PYY and glucose
DIETARY_SUPPLEMENTumami-block
oral application of umami substances as glutamate with and without IMP, collection of blood samples as above
OTHERgastroscopy
gastroscopy with and without oral application of saccharin, tissue samples from th upper duodenum by endoscopy for the detection of taste receptor cells in the upper bowel
Sponsors
Technical University of Munich
German Institute of Human Nutrition
Study design
Allocation
RANDOMIZED
Intervention model
FACTORIAL
Primary purpose
BASIC_SCIENCE
Masking
SINGLE (Subject)
Eligibility
Sex/Gender
ALL
Age
18 Years to 75 Years
Healthy volunteers
Yes
Inclusion criteria
* Healthy persons
Exclusion criteria
* Tumor in history * Pregnancy * Severe illness in history * Bodymass index below 18
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Area under the curve (AUC): changes of expression levels of the sweet , bitter und umami taste receptors in humans in the upper bowel and responses of incretins to receptor agonists and antagonists | three years | measurement of incretins (GIP, GLP-1) and peptide hormones in human plasma after consumption of different sugars , sweeteners , bitter tasting ingredients of vegetables und umami. Measurement of expression levels in human intestinal mucosa before and after receptor stimulation |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| changes in the signalling cascade of the sweet receptor SGLT1 (sodium tranporter 1), changes of receptor expression | 3 years | oral application of sweet tasting substances and antagonists, endoscopic collection of tissue samples, determination of molecular resonses by qRT-PCR and immunohistochemistry |
Other
| Measure | Time frame | Description |
|---|---|---|
| Changes in the Area under the Curve (AUC) for PYY in different bitter tasting substances | 3 years | oral application of sinigrin, lactucopicrin and other extracts of vegetables and umami, getting insight into the different bitter receptors |
Countries
Germany
Outcome results
None listed