Chronic Periodontitis, Periodontal Bone Loss
Conditions
Keywords
Autogenous bone graft, Chronic periodontitis, Enamel matrix derivatives, Gingival crevicular fluid, Periodontal flap surgery
Brief summary
The present study aimed to evaluate the effects of enamel matrix derivatives either alone or combined with autogenous bone graft applied to intrabony defects in chronic periodontitis patients on clinical/radiographic parameters and gingival crevicular fluid transforming growth factor-β1 level and, to compare with open flap debridement. Our hypothesis is to test whether the use of autogenous bone graft and enamel matrix derivative combination in the treatment of intrabony periodontal defects enhance the clinical, radiographic and biochemical parameters in comparison to the use of open flap debridement alone.
Detailed description
The present study aimed to evaluate the effects of enamel matrix derivatives (EMD) either alone or combined with autogenous bone graft (ABG) applied to intrabony defects in chronic periodontitis patients on clinical/radiographic parameters and gingival crevicular fluid (GCF) transforming growth factor-β1 (TGF-β1) level and, to compare with open flap debridement (OFD). A total of 30 deep intrabony defects in 12 patients were randomly treated with EMD+ABG (Combination group), EMD alone (EMD group) or OFD (Control group). Clinical parameters including plaque index, gingival index, bleeding on probing, probing depth, relative attachment level and recession were recorded at baseline and 6 months post-surgery. Intrabony defect fill percentage was calculated on the standardized radiographs. TGF-β1 level was evaluated in GCF just before surgery and 7, 14, 30, 90, 180 days after surgery using ELISA.
Interventions
PROCEDUREOpen Flap Debridement
Open Flap Debridement (Control Group)
BIOLOGICALEmdogainⓇ(Enamel Matrix Derivative)
Open Flap Debridement+Enamel Matrix Derivative
BIOLOGICALEmdogainⓇEnamel Matrix Derivative+Autogenous Bone
Open Flap Debridement+EmdogainⓇ(Enamel Matrix Derivative)+Autogenous Bone
Sponsors
Haner Direskeneli, Prof
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
TREATMENT
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
32 Years to 57 Years
Healthy volunteers
Yes
Inclusion criteria
(a) no systemic diseases as diabetes mellitus or cardiovascular diseases that contraindicated periodontal surgery and could influence the outcome of the therapy; (b) no smoking (c) no medications affecting periodontal tissues; (d) no pregnancy or lactation; (e) a good level of oral hygiene (plaque index \< 1, and full mouth bleeding on probing score \<20% after initial periodontal treatment), (f) compliance with the maintenance programme and (g) presence of at least one intra-bony defect with a probing depth ≥6 mm, radiographic depth of the defect ≥3 mm as detected on the radiographs.
Exclusion criteria
(a) pregnancy or lactating, (b) required an antibiotic premedication, (c) received antibiotic treatment in the previous 6 months, (d) smokers, (e) whose tooth had inadequate amount of attached keratinized gingiva (\<1mm)
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Attachment Gain | 6 months after surgery |
Secondary
| Measure | Time frame |
|---|---|
| Gingival crevicular fluid TGF-β1 level | 1 week, 2 weeks, 4 weeks, 12 weeks and 72 weeks after surgery |
Other
| Measure | Time frame |
|---|---|
| Gingival index | Baseline and 6 months post-surgery |
| Bleeding on probing | Baseline and 6 months post-surgery |
| Probing depth | Baseline and 6 months post-surgery |
| Relative attachment level | Baseline and 6 months post-surgery |
| Recession | Baseline and 6 months post-surgery |
| Intrabony defect fill percentage | Baseline and 6 months post-surgery |
| Plaque index | Baseline and 6 months post-surgery. |
Countries
Turkey (Türkiye)
Outcome results
None listed