Diagnostic Interest of Leukocyte Immunophenotyping Using Multiparameter 8-color Flow Cytometry in Heart Surgery

Status

Completed

Phases

Unknown

Study type

Observational

Source

ClinicalTrials.gov

Registry ID

NCT02216019

Acronym

INFLUX

Enrollment

Registered

2014-08-13

Start date

2013-11-30

Completion date

2014-04-30

Last updated

2014-08-15

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Cardiac Surgery

Keywords

cardiac surgery, infectious complication

Brief summary

In septic patients, Septiflux1 study identified immature granulocytes which may have a diagnostic and prognostic interest. However, with these preliminary results it is not possible to make a difference between what is linked to the infection and what is a consequence of the inflammation which goes along with the septic process. Using flow cytometry, the study of leukocytes in a pure inflammatory model such as cardiopulmonary bypass in heart surgery could make possible to test the diagnostic interest of the immature granulocytes but also to attribute a prognostic value for the occurrence of post-operative infections.

Detailed description

Expression of CD10, CD16, CD24, CD64 on granulocytes, of CD14 and CD16 on monocytes and of CD3 on T lymphocytes. Flow cytometers will all be calibrated the same way using common reagents (known fluorescent calibration beads). Moreover, CD45 and CD3 level of expression on lymphocytes will help to check that the collection of data is standardized between the different centers.

Interventions

OTHERWhole blood

preoparatoire whole blood and postoperative whole blood

Study design

Observational model

COHORT

Time perspective

PROSPECTIVE

Eligibility

Sex/Gender

ALL

Age

18 Years to No maximum

Healthy volunteers

Inclusion criteria

* Patient older than 18 years old * Patient undergoing planned heart surgery with cardiopulmonary bypass

Exclusion criteria

* Pregnancy, * progressive solid cancer, * HIV infection, * history of blood or inflammatory disease, * long-term immunosuppressive treatment, * urgent surgical intervention, * documented preoperative infection

Design outcomes

Primary

Measure	Time frame	Description
Expression of the marker CD64, CD10 and CD16	1 hour	Evolution rate of CD64, CD10 and CD16 in the immediate postoperative cardiac surgery

Secondary

Measure	Time frame	Description
Early onset of infectious complications	7 days	Early onset of infectious complications in the 7 days post-operative

Countries

France

Outcome results

None listed

Source: ClinicalTrials.gov · Data processed: Feb 4, 2026