Blastocyst Time, Cleavage Times, Embryo Compaction, Pronuclear Appearance, Pronuclear Fading
Conditions
Brief summary
The embryokinetics may be a new prognostic factor for choosing the human embryos with the highest implantation potential. In order to identify the factors that may affect the rate of embryo cleavage, there are many studies about morphokinetics and culture medium, oxygen tension, oocyte quality or type of medication used for ovarian stimulation. However there are no published data with respect to the influence of sperm DNA fragmentation in the embryo kinetic.
Interventions
OTHERMACS
Sponsors
IVI Madrid
Study design
Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
36 Years to 38 Years
Healthy volunteers
No
Inclusion criteria
* Patients 36 to 38 years * Fresh sample semen * Minimum 6 oocytes * Semen sample with at least 5 million/ml and at least 30% of progressive motile sperm.
Exclusion criteria
* Patients younger than 36 or older than 38 years old * Frozen semen sample * Less tan 6 oocytes. * Semen samples with less than 5 million / ml or less than 30% of motile sperm.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Time of pronuclear appearance in hours. | The mean time of appearance of pronuclei is 3.3951 hours in our patients. We are going to evaluate pronuclear appearence time in hours in our study groups | We want to study whether differences exist in the time of appearance of pronuclei in those embryos that derived from semen samples from the two study groups the minimum value of the difference to be detected; we said that about 3 hours apart. |
Countries
Spain
Outcome results
None listed