Body Composition, Ectopic Fat, Muscle Mass, Obesity, Overfeeding
Conditions
Keywords
Overfeeding, Weight gain, Liver fat, Pancreatic fat, Ectopic fat, Skeletal muscle, Adipose tissue, Gene expression, DNA methylation, Palmitate, Linoleic acid, Saturated fat, Polyunsaturated fat, Metabolism, Insulin sensitivity
Brief summary
To investigate metabolic and molecular response to fatty acid-specific overfeeding in overweight subjects, in relation to changes in ectopic fat, lean tissue mass and insulin sensitivity
Interventions
DIETARY_SUPPLEMENTPUFA-group
DIETARY_SUPPLEMENTSFA-group
Sponsors
Karolinska Institutet
Uppsala University
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
BASIC_SCIENCE
Masking
QUADRUPLE (Subject, Caregiver, Investigator, Outcomes Assessor)
Eligibility
Sex/Gender
ALL
Age
30 Years to 50 Years
Healthy volunteers
Yes
Inclusion criteria
* Body mass index 25-32
Exclusion criteria
* Type 2 diabetes * Type 1 diabetes * Kidney disease * Liver disease * Abnormal clinical chemistry at screening * Intense physical exercise \> 2 hours per week * Use of statins or drugs affecting energy metabolism * Use of extreme diets
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Change in lean tissue mass | 10 weeks | Assessed by magnetic resonance imaging (MRI) and air displacement plethysmography (BodPod) |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Change in hepatic fat content | 10 weeks | Assessed by magnetic resonance imaging (MRI) |
| Change in visceral adipose tissue (VAT) | 10 weeks | Assessed by magnetic resonance imaging (MRI) |
| Change in total adipose tissue | 10 weeks | Assessed by magnetic resonance imaging (MRI) |
| Change in insulin sensitivity | 10 weeks | Assessed by oral glucose tolerance test (OGTT) |
| Change in plasma lipids | 10 weeks | Plasma cholesterol, low-density lipoprotein, high-density lipoprotein, triglycerides, apolipoprotein B, apolipoprotein A1, free fatty acids, glycerol, proprotein convertase subtilisin/kexin type 9 |
| Change in circulating inflammation and coagulation markers | 10 weeks | Plasma tumor necrosis factor-receptor 2, Interleukin receptor 1 antagonist, C-reactive protein, fibrinogen, adhesion molecules |
| Change in pancreatic fat | 10 weeks | Assessed by MRI |
Other
| Measure | Time frame | Description |
|---|---|---|
| Circulating hormones and molecular analyses in muscle and fat tissue | 10 weeks | Serum hormones including prolactin, tyroxin, T3, cortisol, growth hormone, Insulin growth factor-1. Fat cell morphology assessment in fat tissue, and gene and protein expression, DNA methylation analyses in fat and muscle tissue, palmitate tracer uptake in liver using positron emission tomography |
Countries
Sweden
Outcome results
None listed