Obesity
Conditions
Keywords
Nighttime Eating, Protein, Obesity, Lipolysis, Metabolism
Brief summary
Single macronutrients will be consumed before sleep by obese men. Overnight lipolysis will be measured with the microdialysis technique by measuring glycerol concentration and blood flow in abdominal adipose tissue. Morning fat oxidation will be measured with indirect calorimetry. The investigators hypothesize that carbohydrate intake will blunt the overnight lipolysis rate compared to casein protein and a non-caloric placebo. The investigators also hypothesize that casein and placebo will not be significantly different from each other in terms of overnight lipolysis.
Interventions
OTHERCasein Protein
The effect of casein protein consumed within 30 minutes of sleep on overnight lipolysis and next morning metabolism and appetite will be examined
OTHERNon-caloric placebo
The effect of a non-caloric placebo consumed within 30 minutes of sleep on overnight lipolysis and next morning metabolism and appetite will be examined
Sponsors
Florida State University
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
TREATMENT
Masking
DOUBLE (Subject, Investigator)
Eligibility
Sex/Gender
MALE
Age
18 Years to 45 Years
Healthy volunteers
Yes
Inclusion criteria
* Healthy, sedentary overweight or obese (BMI ≥25 kg/m\^2; body fat ≥ 25% ) men
Exclusion criteria
* Men that have been exercising for more than 2 days per week for more than 45 minutes per session (within the past 6 months), have uncontrolled hypertension (Blood Pressure (BP)\>160/100 mmHg, diagnosed cardiovascular disease, stroke, diabetes, thyroid or kidney dysfunction, take cholesterol medication, or have any allergies to milk products will be excluded. Smokers will also be excluded.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Lipolysis | Change from day 1 to day 2 to day 3 of testing | Measuring lipolysis using the microdialysis technique. Samples will be collected at various time intervals Vial 1: 30 minute; 2.0 uL/min flow rate Vial 2: 4 hours; 0.3 uL/min flow rate Vial 3: Overnight; 0.3 uL/min flow rate (before taking nighttime supplement) Vial 4: Morning sample #1; 2.0 uL/min flow rate (upon waking) Vial 5: Morning sample #2; 2.0 uL/min flow rate (arrive to lab) Blood flow will be measured using the Ethanol technique |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Resting Metabolic Rate (RMR) | Change from day 1 to day 2 to day 3 | RMR will be measured with indirect calorimetry using the ventilated hood technique |
Other
| Measure | Time frame | Description |
|---|---|---|
| Appetite | Change from Baseline to day 1, 2, and 3 | Appetite will be measured by visual analog scales |
| Blood hormones | Change from Baseline to day 1, 2, and 3 | Hormones related to appetite and metabolism will be examined. |
Countries
United States
Outcome results
None listed