Childhood Obesity, Insulin Resistance
Conditions
Keywords
children, obesity, adipose tissue dysfunction, inflammation, macrophage infiltration, insulin resistance, dyslipidemia
Brief summary
In this study the investigators hypothesize that pathological alterations in adipose tissue biology already occur during the development and progression of obesity in children and adolescents. The investigators aim to identify and characterize mechanisms and molecular targets that affect the development of adipose tissue and ensuing obesity in childhood and adolescence.
Detailed description
The investigators aim to identify how adipose tissue dysfunction in childhood contributes to the development of obesity and related comorbidities and to characterize factors that play a role in the development of adipose dysfunction in children. The investigators will employ a translational approach which is based on the characterization of adipose tissue biology in samples of children to determine alterations leading to adipose tissue dysfunction. These include assessment of the composition (including BAT), remodeling, function, metabolism and inflammation of adipose tissue as well as the adipokine profile. For the assessment of clinical relevance, these experimental data will then be correlated with the clinical phenotype. Finally, we aim to identify factors responsible for early adipose tissue dysfunction and characterize them for their clinical and functional relevance.
Interventions
None listed
Sponsors
University of Leipzig
Study design
Observational model
COHORT
Time perspective
CROSS_SECTIONAL
Eligibility
Sex/Gender
ALL
Healthy volunteers
Yes
Inclusion criteria
* body weight: 3000 g - 180 kg * suitable surgical procedure * signed informed consent by the guardians and the study participant, if older 12 years
Exclusion criteria
* severe chronic and inflammatory diseases * acute or chronic infections * oncological diseases * clotting disorders * complications during surgical procedure * drug treatment * nil by mouth
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Adipose tissue dysfunction | 10 years | Adipose tissue dysfunction is assessed by evaluation of adipocyte size and number (hypertrophy vs. hyperplasia), adipocyte proliferation and differentiation, (lipid) cellular metabolism, inflammation, gene expression, fibrosis, and others; the association of AT dysfunction with clincial phenotype will be assessed |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Presence of brown adipose tissue (BAT) | 10 years | Adipose tissue samples will be evaluated for the presence of BAT on histological and molecular level and association with clinical phenotype will be investigated |
| Inflammation of adipose tissue | 10 years | Inflammation will be assessed by evaluating macrophage infiltration on histological and molecular expression level. Also, association with clinical phenotype will be assessed. |
Countries
Germany
Contacts
Primary ContactAntje Körner, Prof. Dr.
Backup ContactRobert Stein, MD
Outcome results
None listed