Iron Absorption
Conditions
Keywords
Non-heme iron absorption; HFE
Brief summary
The two specific aims of this study are: 1) to assess the impact of a genetic variant on iron status; 2) to assess the impact of a genetic variant on non-heme absorption among Asian women. The investigators hypothesize that the genetic variant could enhance iron status and iron absorption in Asian women.
Detailed description
To determine the HFE genotype of female Asian study volunteers, we collected venous blood samples and extracted DNA from these blood samples. HFE genotype was determined as CC, CT, and TT and used to assess possible differences in iron status as a function of genotype. To measure the impact of genotype on non-heme Fe absorption, a sub-group of women with the CC (n=10) or TT (n=11) genotype were invited to return for an Fe absorption study. Each volunteer consumed stable 57Fe (as ferrous sulfate). Two weeks after ingesting this tracer, a blood sample was collected from each woman and the amount of non-heme (57Fe) iron incorporated into red blood cells was measured with magnetic sector thermal ionization mass spectrometry. Possible associations between iron status and iron absorption as a function of genotypes were explored. Data from this study will provide information that aims to improve human health by better understanding the iron requirements of individuals with different genetic background.
Interventions
None listed
Sponsors
Cornell University
Study design
Observational model
CASE_ONLY
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
FEMALE
Age
18 Years to 35 Years
Healthy volunteers
Yes
Inclusion criteria
* non-pregnant and between the ages of 18\ 35 y * East Asian descent with both maternal and paternal grandparents from East Asia, including China, Japan, South Korea, Vietnam, Thailand, Malaysia and Singapore
Exclusion criteria
* taking or planing to take any vitamin or mineral supplements during the study period * pre-existing medical problems that might impact inflammation or Fe status, including malabsorption, blood disorders, ulcers, joint diseases and asthma * taking any prescribed medications known to affect iron homeostasis
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Non-heme Iron absorption in women with 2 HFE genotypes | one month for screening with genotype then 2 weeks for iron absorption study | Women donated a blood sample for genotyping. Women with particular HFE genotypes were invited to return for an absorption study. Two weeks after women consume the iron tracer, a blood sample was obtained to measure the amount of iron absorbed. |
| Hemoglobin | Up to 2 days after the participant's visit to the laboratory | At the participant's visit to the laboratory, a 10 mL blood sample was collected. The blood sample was used for determining the concentration of hemoglobin. |
| Serum Ferritin | Up to 6 months after the participant's visit to the laboratory | At the participant's visit to the laboratory, a 10 mL blood sample was collected. The blood sample was used for determining the concentration of serum ferritin. |
| Serum transferrin receptor | Up to 6 months after the participant's visit to the laboratory | At the participant's visit to the laboratory, a 10 mL blood sample was collected. The blood sample was used for determining the concentration of serum transferrin receptor. |
| Genotype at SNP rs9366637 | 2 weeks after the participant's visit to the laboratory | At the participant's visit to the laboratory, a 10 mL blood sample was collected. The blood sample was used for determining genotype. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Vitamin B-12 | Up to 6 months after the participant's visit to the laboratory | At the participant's visit to the laboratory, a 10 mL blood sample was collected. The blood sample was used for determining the concentration of vitamin B-12. |
| C-reactive protein | Up to 6 months after the participant's visit to the laboratory | At the participant's visit to the laboratory, a 10 mL blood sample was collected. The blood sample was used for determining the concentration of C-reactive protein. |
| Folate | Up to 6 months after the participant's visit to the laboratory | At the participant's visit to the laboratory, a 10 mL blood sample was collected. The blood sample was used for determining the concentration of folate. |
Countries
United States
Outcome results
None listed