Basic Research Study to Examine the Relation Between Muscle Insulin Resistance and Lipid Production in the Liver.

Lipid and Carbohydrate Metabolism: Effects of Exercise

Status

Completed

Phases

Study type

Interventional

Source

ClinicalTrials.gov

Registry ID

NCT02196051

Enrollment

Registered

2014-07-21

Start date

2008-01-01

Completion date

2012-01-30

Last updated

2024-08-15

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Insulin Resistance

Keywords

Insulin resistance, Exercise, de novo lipogenesis, NAFLD, Magnetic Resonance Spectroscopy

Brief summary

The purpose of this study was to examine the pathophysiology of insulin resistance and the relation to the metabolic syndrome in humans.

Detailed description

In this study insulin resistant (IR), young offspring of parents with type 2 diabetes (18-46 years) and healthy, lean elderly individuals (65-90 years) underwent 1H/13C MRS measurements of liver and muscle lipid and glycogen content before and after a high carbohydrate meal challenge. Hepatic de novo lipogenesis was determined as the incorporation of deuterium, from deuterium labeled water (2H2O), into plasma triglycerides 5. These metabolic studies were performed in 2 sets: Study Part 1: Resting, Study Part 2: After 1 bout of 45 minutes exercise. The study has been completed and published as two papers: August 16, 2011 and July 24, 2012.

Interventions

OTHERone bout of 45 minutes exercise

45 minutes of exercise on an elliptical exercise machine

Study design

Allocation

Intervention model

SINGLE_GROUP

Primary purpose

BASIC_SCIENCE

Masking

NONE

Eligibility

Sex/Gender

ALL

Age

18 Years to 90 Years

Healthy volunteers

Yes

Inclusion criteria

* Body Mass Index \< 29 kg/m2 * No regular exercise routine * Non-smokers * Absence of systemic and organ disease.

Exclusion criteria

* Hematocrit \< 35 vol% * History of claustrophobia * Ferromagnetic implants * Regular exercise regimen * Not weight stable * Any significant hematological, neurological, musculoskeletal or endocrine disease (including diabetes mellitus) * Any significant cardiovascular condition, including confirmed CAD and prior cardiovascular events * Taking coumadin as anticoagulant therapy.

Design outcomes

Primary

Measure	Time frame	Description
change in de novo lipogenesis	Baseline	At the baseline assessment, de novo lipogenesis was assessed after a high carbohydrate meal and a drink of heavy water. Muscle glycogen and lipid contents were measured pre- and post meal using 1H/13C MRS and de novo lipogenesis was assessed from the heavy water incorporation into blood lipid in samples collected overnight.

Countries

United States

Outcome results

None listed

Source: ClinicalTrials.gov · Data processed: Feb 4, 2026