Aortic Valve Stenosis
Conditions
Keywords
Microparticles, Severe aortic stenosis, Calcified aortic stenosis, transcatheter aortic valve replacement
Brief summary
Microparticles are small cell fragments that can induce fat plaques, calcification and formation of thrombus. They can be released through multiple stimulations, but also the high flow of blood through partially obstructed aortic valves. In patients with severely obstructed aortic valves the investigators hypothesize that microparticles levels will be elevated and that they will go down after percutaneous treatment of the valves.
Detailed description
Microparticle (MPs) levels and subtypes according to endothelial cell, macrophage and platelet markers will be measured by flow-cytometry using appropriate fluorochromes. Patient baseline (including measures of severity of aortic stenosis and its calcification), procedural data, and clinical evolution will be collected. Inflammatory markers will be measured, which along with clinical date will be tested for correlation with microparticle levels. Patients MPs will be measured before and after percutaneous treatment of the severe aortic stenosis.
Interventions
OTHERMicroparticle levels
Microparticle levels will be evaluated with flow cytometry and nanoparticle tracking analysis
Sponsors
InCor Heart Institute
Study design
Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
DIAGNOSTIC
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
No
Inclusion criteria
* Clinical indication to be treated with transcatheter aortic valve replacement.
Exclusion criteria
* Non native aortic valve * Moderate aortic regurgitation * The following complications following valve implantation: patient instability, need for vasoactive pressors, sepsis and severe infections, stroke.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Difference in microparticle levels | Day 5 | Difference of total microparticle levels before and after treatment of patients with percutaneous aortic valve replacement |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Endothelial microparticle levels | Day 5 | Difference of endothelial microparticle levels before and after treatment of patients with percutaneous aortic valve replacement |
| Macrophage microparticle levels | Day 5 | Difference of macrophage microparticle levels before and after treatment of patients with percutaneous aortic valve replacement |
| Platelet microparticle levels | Day 5 | Difference of platelet microparticle levels before and after treatment of patients with percutaneous aortic valve replacement |
Countries
Brazil
Outcome results
None listed