Mitral Stenosis, Rheumatic Heart Disease
Conditions
Keywords
Rheumatic heart disease, Acute rheumatic fever, Group A streptococcal disease, Streptococcus pyogenes, Genetics, Oceania
Brief summary
The purpose of this study is to investigate whether there are genetic differences between patients with rheumatic heart disease and members of the general population.
Detailed description
The investigators will micro-array genotype approximately 300,000 single nucleotide polymorphisms (SNP) using DNA samples from patients with rheumatic heart disease (cases) from New Caledonia and Fiji, and members of the general population (controls) from New Caledonia, Vanuatu and Fiji. The investigators will perform standard quality control checks on the SNP data using measures such as call rate, heterozygosity, duplication and relatedness, and exclude variants on the basis of deviation from Hardy-Weinberg equilibrium and minor allele frequency. We will also impute variants not present on the micro-array with reference to the latest release of 1000 Genomes data and whole-genome sequence data from sixty Melanesian individuals from New Caledonia from the phenotypic extremes in this study. The investigators will conduct a discovery analysis in using a genome-wide association study approach focusing on Oceanic cases and controls from the Francophone nations of New Caledonia and Vanuatu. This analysis will be corrected for bias due to population stratification using the Linear Mixed Model (LMM) and consider additive, dominant and recessive genetic models. The investigators will then perform LMM association testing for variants with P-value in the discovery analysis less than 1x10\^-5 in Oceanic cases and controls from Fiji and combine the association statistics by fixed-effects meta-analysis. The investigators will consider variants with significant effects in the same direction in discovery and replication analyses with combined P-value less than 1x10\^-8 to have replicated. Unless there is clear evidence that associated variants are specific to Oceanic populations, further replication analyses for associated variants in cases and controls of Indian Descent from Fiji, as well as individuals of other and admixed ethnicities from both Fiji and New Caledonia. Recruitment completed in December 2013. After receipt of funding from the British Heart Foundation, genotyping and analysis will begin in July 2014.
Interventions
None listed
Sponsors
Ministry of Health, Fiji
Fiji National University
Institut National de la Santé Et de la Recherche Médicale, France
Institut Necker Enfants Malades
Centre Hospitalier Territorial de Nouvelle-Calédonie
University of Melbourne
University of Oxford
Study design
Observational model
CASE_CONTROL
Time perspective
RETROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
5 Years to No maximum
Healthy volunteers
Yes
Inclusion criteria
1. Cases 1. Inclusion Criteria: * Diagnosis of rheumatic heart disease based on one of: * World Heart Federation definite echocardiographic criteria * World Heart Federation borderline echocardiographic criteria and history of acute rheumatic fever * Mitral stenosis with valve area less than 2.0 cm2 * Previous surgery for rheumatic heart disease 2.
Exclusion criteria
* Age less than five years * Inability to give consent 2. New Controls 1. Inclusion criteria: * Healthy individual living in a community where cases were identified 2.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Rheumatic heart disease diagnosis | At enrolment | Diagnosis of rheumatic heart disease as layout in enrolment criteria for cases. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Mitral stenosis diagnosis | At enrolment | History or echocardiographic diagnosis of mitral stenosis |
Countries
Fiji, New Caledonia, United Kingdom
Outcome results
None listed