Insulin Resistance
Conditions
Brief summary
Previous research has shown that indulging in 50% more calories than required for as little as 3 days can significantly impact markers of metabolic health in lean and overweight individuals. Here, the investigators will determine if 3 brief sessions of high-intensity interval training can mitigate the adverse consequences of 7 days high-fat overfeeding in sedentary, overweight males.
Interventions
OTHERWingate HIT
3 sessions of Wingate based High-Intensity Interval Training (6-8 x 15 second all out sprints on a cycle ergometer interspersed by 2 minutes recovery)
OTHERModified HIT
3 sessions of Modified High-Intensity Interval Training (8 x 60 second sprints at approximately 90-95% VO2 peak on a cycle ergometer interspersed by 60 seconds recovery)
OTHERHigh-fat overfeeding
7 days of high-fat overfeeding (50% excess calories)
Sponsors
Royal Adelaide Hospital
University of South Australia
University of Adelaide
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
PREVENTION
Masking
NONE
Eligibility
Sex/Gender
MALE
Age
18 Years to 45 Years
Healthy volunteers
Yes
Inclusion criteria
* Sedentary (no more than 2 sessions of structured moderate intensity aerobic exercise or resistance training per week) * Overweight (BMI 25-29.9 kg/m2)
Exclusion criteria
* Personal history of any major illness (i.e. cardiovascular disease, diabetes, hypertension, cancer, renal/liver impairments, major psychiatric disorders, eating disorders etc.) * History of chest pain (either at rest or during exercise.) * Abnormal resting ECG at screening visit * Chronic use of any prescribed or non-prescribed medications (i.e. anti-hypertensive, statins, metformin, anti-inflammatories, antidepressants etc.) * Blood pressure or blood lipids outside of reference ranges * Serum ferritin \<30ng/mL * Uncontrolled asthma, current fever, or upper respiratory infection * Current intake of \> 140g alcohol/week * Current smokers of cigarettes/cigars/marijuana * Current intake of any illicit substance * Experience claustrophobia in confined spaces * Donated blood in the past 3 months * Migraines * Unable to comprehend study protocol * Unable to perform exercise on a cycle ergometer at second screening visit
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Change in glucose metabolism | 1 week | Changes in insulin and glucose area under the curve assessed via 75g Oral Glucose Tolerance Test. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Change in plasma levels of cytokines | 1 week | Change in plasma levels of adiponectin, irisin, sex hormone-binding globulin and c-reactive protein |
Other
| Measure | Time frame | Description |
|---|---|---|
| Change in mitochondrial metabolism | 1 week | Changes in citrate synthase activity, mitochondrial DNA content and protein levels of PGC-1a |
| Change in resting energy expenditure | 1 week | Changes in resting energy expenditure measured by metabolic cart |
| Change in cognitive function | 1 week | Changes in cognitive function, assessed by a series of computer tasks involving vigilant attention and speed processing. |
Countries
Australia
Outcome results
None listed