Assisted Reproductive Technology, Freezing/Thawing Procedure, Prolonged Culture Procedure
Conditions
Brief summary
For this project, 4 groups of 38 infants have been created so as to evaluate the impact of Assisted-Reproduction Technology (ART) and more particularly the two procedures classically used in ART: the freezing/thawing of embryos and prolonged embryo culture (five days of culture in appropriate in vitro conditions to allow development of the embryo) in contrast with shorter culture of only two days. Participation consists in accepting samples to be taken at the birth of the infant: 1. from the umbilical cord. 2. from the placenta.
Interventions
OTHERSample of cord blood
OTHERSample of placenta
Sponsors
Centre Hospitalier Universitaire Dijon
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to 50 Years
Healthy volunteers
Yes
Inclusion criteria
* Patients who have provided written informed consent, * Patients with national health insurance cover, * Maternal age at conception between 20 and 43 years, * Paternal age at conception between 18 and 50 years, * Singleton pregnancy
Exclusion criteria
---Maternal disease: * pulmonary, cardiac * renal and metabolic diseases (including type 1 and 2 diabetes prior to the pregnancy) * systemic inflammatory diseases * hypertension * neurological diseases * chronic hepatitis B or C * HIV infection
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Analysis of the expression of several genes subjected to imprinting (H19, IGF2, IGF2R, MEST, PEG3, SNRPN, IGF2, KCNQ1, GRB10 and PLAGL1) | Baselines | DNA / RNA extraction |
| Analysis of the expression of 3 transposable elements (HERV-K, LINE-1 and ALU) | Baselines | DNA / RNA extraction |
| Level of methylation and the level of expression of Genomic Imprinting (GI) and Transposable Elements (TE) | Baselines | DNA / RNA extraction |
Countries
France
Outcome results
None listed