Medical Termination of Pregnancy, Voluntary Termination of Pregnancy
Conditions
Keywords
Male foetuses of 10 to 37 weeks
Brief summary
This is a single-centre study over 36 months for the inclusion of 30 patients. The selection and inclusion of patients will take place according to the criterion of Medical Termination of Pregnancy at 4 different gestational ages (8-12 weeks of gestation (WG)), 13-16 WG, 17-23 WG and 25-35 WG. The tissue analysed will principally be the gonads, other somatic tissue will also be systematically harvested in parallel (liver and psoas muscle) for control purposes. The gonads will be subjected to molecular biology analyses destined to quantify the expression of transposons and their inhibitors.
Interventions
OTHERsurgical biopsies
Sponsors
Centre Hospitalier Universitaire Dijon
Study design
Observational model
CASE_ONLY
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
MALE
Age
10 Weeks to 37 Weeks
Healthy volunteers
No
Inclusion criteria
* Couple or mother who has provided written informed consent * Foetus between 10 and 37 weeks of amenorrhea (WA) * Male foetus * Foetus obtained following medical termination of pregnancy or voluntary termination of pregnancy
Exclusion criteria
* persons without national health insurance cover * foetus presenting aneuploidy * foetus presenting a malformation of the genital organs
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Spatio-temporal analysis of the activity of TEs by RT-qPCR and immunostaining | Baselines |
Secondary
| Measure | Time frame |
|---|---|
| Promoter methylation profile by pyrosequencing | Baselines |
Other
| Measure | Time frame |
|---|---|
| Spatiotemporal analysis piARN way: RT qPCR, Small RNA Seq, and immunoprecipitation of proteins PIWI | Baselines |
Countries
France
Contacts
Primary ContactPatricia FAUQUE
Outcome results
None listed