Blood Platelet Disorders, Cardiac Function Impaired, Cardiovascular Diseases, Menopause, Metabolic Diseases
Conditions
Keywords
premenopausal, postmenopausal, cardiovascular, exercise training, vascular function, cardiac function, cardiac morphology, health
Brief summary
The hypothesis of the present study is that physical training can oppose detrimental changes in cardiovascular and metabolic health associated with estrogen loss after menopause.
Interventions
BEHAVIORALSpinning
After the baseline studies the pre and postmenopausal women will be randomized to spinning training groups and the intervention will last for 3 months. The spinning group will spin three times per week under guidance of a trained instructor. The training is heart rate monitored.
Sponsors
Glostrup University Hospital, Copenhagen
Rigshospitalet, Denmark
University of Copenhagen
Study design
Allocation
NON_RANDOMIZED
Intervention model
PARALLEL
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
FEMALE
Age
45 Years to 57 Years
Healthy volunteers
Yes
Inclusion criteria
* Premenopausal (45-53 years) OR postmenopausal 50-57 years * BMI\> 18.5 and \<30 * Sedentary (no regular physical activity the last 2 years) * Plasma measurements * Premenopausal: estradiol\> 0.20 nmol/l, Follicle stimulating hormone \< 22 IU/l and progesterone \>2.5 nmol/l * Postmenopausal: estradiol \< 0.20 nmol/l, Follicle stimulating hormone \> 22 IU/l and progesterone 0.3-2.5 nmol/l
Exclusion criteria
* Smoking * Excessive alcohol intake * Regular medication including hormone replacement treatment * Chronic disease
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Platelet function | Baseline comparison and changes from baseline to 3 months | Platelet aggregation assay of whole blood and platelet rich plasma |
| Cardiac structure and function | Baseline comparison and changes from baseline to 3 months | Magnetic resonance imaging and transthoracic echocardiography |
| Leg vascular function | Baseline comparison and changes from baseline to 3 months | Measurements of leg blood flow by ultrasound doppler during exercise and during femoral arterial infusion of vasodilators |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Genomic profile in blood samples | Baseline comparison and changes from baseline to 3 months | Genomic analysis of parameters for vascular and metabolic health |
| Insulin sensitivity and glycaemic control | Baseline comparison and changes from baseline to 3 months | Fasting plasma glucose analysis and oral glucose tolerance test |
| Gene expression of proteins in muscle samples | Baseline comparison and changes from baseline to 3 months | Gene analysis of relevance for vascular and metabolic function |
| Health related parameters in blood | Baseline comparison and changes from baseline to 3 months | Plasma lipid status and other variables related to vascular health |
| Arterial blood pressure | Baseline comparison and changes from baseline to 3 months | — |
| Expression of proteins in muscle samples | Baseline comparison and changes from baseline to 3 months | Western Blot analysis of proteins of relevance for vascular function and metabolism |
| Maximal aerobic effect | Baseline comparison and changes from baseline to 3 months | Maximal oxygen uptake during an incremental cycle ergometry test with gas analysis |
Other
| Measure | Time frame | Description |
|---|---|---|
| Anthropometry | Baseline comparison and changes from baseline to 3 months | Dual x-ray absorptiometry, height, weight, waist and hip circumference |
| Activity measure | Baseline comparison and changes from baseline to 3 months | Activity measure for one week by accelerometer |
Countries
Denmark
Outcome results
None listed