Metabolic Syndrome, Type 2 Diabetes, Critical Illness
Conditions
Brief summary
Bed rest produces insulin resistance in healthy volunteers. In this study the investigators aim to investigate the effect of 8 days bed rest on the incretin effect and how alternate daily fasting affects cognitive function and the insulin resistance produced by bed rest. The subjects will be randomized to either 3 meals a day (isocaloric diet), alternate daily fasting or one meal/day every second day (25% of daily calorie need) and four meals/day every second (175% of daily calorie need). The investigators hypothesize: 1. Bed rest reduces the incretin effect 2. Alternate daily fasting improves the cognitive function (memory and concentration) compared to isocaloric diet 3. Alternate daily fasting reduces insulin resistance produced by bed rest compared to isocaloric diet
Detailed description
All subjects will undergo 8 days of bedrest. Outcome measures will be performed before, under and immediately after the bed rest period. The individual study period will be 10 days in total.
Interventions
OTHERBed rest
8 days of full bed rest
OTHEROGTT
Oral glucose tolerance test with 75 g of glucose before and after bed rest
OTHERIVGTT
Intravenous glucose tolerance test mimicking the glucose profile from the corresponding OGTT
OTHERCognitive testing
Daily testing of memory and concentration using standardized tests
OTHERMuscle and fat biopsies
Biopsies will be obtained before and after bed rest
DXA scan to evaluate fat and muscle distribution before and after bed rest
OTHERMRI
Functional MRI to evaluate cognitive function and abdominal MRI to evaluate visceral fat before and after bed rest
Sponsors
Rigshospitalet, Denmark
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
MALE
Age
18 Years to 35 Years
Healthy volunteers
Yes
Inclusion criteria
* BMI\<25kg/m2 * VO2 max normal for age
Exclusion criteria
* Tobacco smoking * Alcohol ingestion \> 14 units per week * Diabetes in nearby relatives * Resection of the small intestine * History of gastric bypass surgery * Risk of deep venous thrombosis * Female
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Incretin effect | 24 weeks | Measured by Insulin levels during Oral glucose tolerance test (OGTT) and Intravenous glucose tolerance test (IVGTT) |
| Cognitive function | 18 months | Evaluated by cognitive testing and functional Magnetic Resonance imaging (MRI) |
| Insulin resistance | 12 weeks | Evaluated by insulin resistance indexes, HOMA-IR and Matsuda index |
Countries
Denmark
Outcome results
None listed